Spheroid three-dimensional culture enhances Notch signaling in cardiac progenitor cells. (11th September 2017)

• Record Type: Journal Article
• Authors: Mauretti, Arianna; Rossi, Fabrizio; Bax, Noortje A. M.; Miano, Carmen; Miraldi, Fabio; Goumans, Marie José; Messina, Elisa; Giacomello, Alessandro; Bouten, Carlijn V. C.; Sahlgren, Cecilia
• Is Part Of: MRS communications. Volume 7:Number 3(2017)
• Page Start: 496
• Page End: 501
• Publication Date: 2017-09-11
• Subjects: Materials -- Periodicals; 620.11
• DOI: 10.1557/mrc.2017.82
• Languages: English
• ISSNs: 2159-6859

Abstract

Abstract: Abstract : Cardiac progenitor cells (CPCs) are a promising candidate for cardiac regeneration, and the interaction between CPCs and their microenvironment can influence their regenerative response. Notch signaling plays a key role in cell fate decisions in the developing and adult heart. Here, we investigated the effect of three-dimensional (3D) spheroid culture, as a model of the 3D microenvironment, on Notch in fetal and adult human CPCs, under room air (20%) and physiological (5%) oxygen tension. Notch signaling is enhanced in 3D spheroids; spheroid culture under 5% O2 further increases Notch signaling enhancement, and might ultimately improve the regenerative potential of CPCs.