Engineering the bacterial endophyte Burkholderia pyrrocinia JK-SH007 for the control of lepidoptera larvae by introducing the cry218 genes of Bacillus thuringiensis. Issue 6 (2nd November 2017)

• Record Type: Journal Article
• Authors: Li, Yang; Wu, Choufei; Xing, Zhong; Gao, Bingli; Zhang, Liqin
• Is Part Of: Biotechnology, biotechnological equipment. Volume 31:Issue 6(2017)
• Page Start: 1167
• Page End: 1172
• Publication Date: 2017-11-02
• Subjects: Bacillus thuringiensis -- cry218 gene -- expression vector -- insecticidal activity; Biotechnology -- Periodicals; Biotechnology -- Periodicals; Biotechnology -- instrumentation -- Periodicals; Periodicals; 660.605
• DOI: 10.1080/13102818.2017.1379361
• Languages: English
• ISSNs: 1310-2818

Abstract

ABSTRACT: To expand the usage of endophytes in agriculture and in forestry, the insecticidal gene cry218 of Bacillus thuringiensis was introduced into a poplar bacterial endophyte Burkholderia pyrrocinia JK-SH007. The cry218 gene was cloned by polymerase chain reaction (PCR) and was inserted into a PHKT2 expression vector that was introduced into the bacterial endophyte JK-SH007. By using sodium dodecyl sulphate polyacryl amide gel electrophoresis (SDS-PAGE) and western blotting, we confirmed that the engineered bacterial endophyte was successfully constructed, and it harboured insecticidal function after the bioassay in planta . The toxicity of the expressed insecticidal protein was analysed on second instar silkworm. The regression equation showed that the median lethal concentration (LC50 ) of the insecticidal protein was 0.77 (0.57–1.04) g/L at 72 h. The insecticidal bacteria genetically modified in this study have laid the foundation for further exploitation of biocontrol bacteria.