Upregulated glutathione transferase omega-1 correlates with progression of urinary bladder carcinoma. (2nd November 2017)

• Record Type: Journal Article
• Authors: Djukic, Tatjana; Simic, Tatjana; Pljesa-Ercegovac, Marija; Matic, Marija; Suvakov, Sonja; Coric, Vesna; Dragicevic, Dejan; Savic-Radojevic, Ana
• Is Part Of: Redox report. Volume 22:Number 6(2017)
• Page Start: 486
• Page End: 492
• Publication Date: 2017-11-02
• Subjects: Glutathione transferase omega 1 -- glutathione -- glutathionylation -- transitional cell carcinoma -- uroepithelium; Free radicals (Chemistry) -- Pathophysiology -- Periodicals; Oxidation, Physiological -- Periodicals; 541.224
• DOI: 10.1080/13510002.2017.1299909
• Languages: English
• ISSNs: 1351-0002

Abstract

ABSTRACT: Objectives : Newly discovered glutathione transferase omega 1 (GSTO1-1) plays an important role in the glutathionylation cycle, a significant mechanism of protein function regulation. GSTO1-1 expression pattern has not been studied in transitional cell carcinoma (TCC), as yet. Methods : A total of 56 TCC tumor and corresponding non-tumor specimens were investigated. Glutathione content and thioltransferase activity were measured spectrophotometrically. Protein-glutathione mixed disulfides were measured fluorimetrically. GSTO1-1 expression was determined by immunoblot and qPCR. Immunoprecipitation with GSTO1-1 antibody was followed by immunoblot using anti-GSTO1, GSTP1, c-Jun, JNK, Akt, phospho-Akt, and ASK1 antibody, while for the total S-glutathionylation levels non-reducing electrophoresis was performed. Results : The contents of reduced glutathione and thioltransferase activity were significantly increased in tumor compared to non-tumor tissue. The increased GSTO1 expression in tumor tissue showed clear correlation with grade and stage. However, decreased total protein glutathionylation level in tumor compared to non-tumor samples was found. Immunoprecipitation has shown an association of GSTO1-1 with GSTP1, Akt, phospho-Akt, and ASK1 proteins. Conclusions : GSTO1 deglutathionylase activity suggests its potential important role in redox perturbations present in TCC. Increased GSTO1-1 expression might contribute to TCC development and/or progression supporting the notion that GSTO1-1 may be a promising novel cancer target.