Agrobacterium tumefaciens-mediated transformation of drumstick (Moringa oleifera Lam.). Issue 6 (2nd November 2017)

• Record Type: Journal Article
• Authors: Zhang, Junjie; Lin, Mengfei; Chen, Hanbin; Chen, Xiaoyang
• Is Part Of: Biotechnology, biotechnological equipment. Volume 31:Issue 6(2017)
• Page Start: 1126
• Page End: 1131
• Publication Date: 2017-11-02
• Subjects: Moringa oleifera -- Agrobacterium tumefaciens -- transformation; Biotechnology -- Periodicals; Biotechnology -- Periodicals; Biotechnology -- instrumentation -- Periodicals; Periodicals; 660.605
• DOI: 10.1080/13102818.2017.1368415
• Languages: English
• ISSNs: 1310-2818

Abstract

ABSTRACT: Drumstick ( Moringa oleifera Lam.) is an industrially important tree species. Due to the availability of an efficient regeneration system, the present study was undertaken to develop a reliable and stable Agrobacterium tumefaciens -mediated transformation system for the genetic improvement of drumstick. A. tumefaciens strain EHA105, harbouring the plasmid pCAMBIA1301 containing the hygromycin phosphotransferase II and ß -glucuronidase genes driven by the CaMV 35S promoter, was used to establish a transformation system in drumstick. The use of vacuum infiltration-assisted Agrobacterium infection (excluding a pre-culture step) and co-cultivation for two days significantly increased the transformation frequency. Genomic integration and transgene expression were confirmed by β-glucuronidase (GUS) assays, polymerase chain reaction (PCR), Southern blot analysis and real-time quantitative PCR (RT-qPCR). This transformation protocol provides a basis for the future development of genetic engineering techniques to improve the performance of drumstick.