The modular synthesis of multivalent functionalised glycodendrons for the detection of lectins including DC-SIGN. Issue 72 (22nd September 2017)

• Record Type: Journal Article
• Authors: Munneke, Stefan; Kodar, Kristel; Painter, Gavin F.; Stocker, Bridget L.; Timmer, Mattie S. M.
• Is Part Of: RSC advances. Volume 7:Issue 72(2017)
• Page Start: 45260
• Page End: 45268
• Publication Date: 2017-09-22
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/c7ra08872h
• Languages: English
• ISSNs: 2046-2069

Abstract

Abstract : A modular approach towards glycoconjugate probes allows for the versatile synthesis of dendrons with reporter groups and glycans of choice. Abstract : Glycodendrons are excellent tools for the biological evaluation of lectins. Herein, we present the expedient and efficient synthesis of a versatile second generation dendron scaffold using double exponential growth methodology. The dendron scaffold can be rapidly functionalised, as illustrated by the conjugation of the dendron scaffold to biotin or a fluorescent probe, followed by oxyamine glycan conjugation to the glycan of choice. The use of a fluorescent Lewis X glycodendron for the detection of the C-type lectin DC-SIGN on macrophages is then demonstrated.