The fluorescently responsive 3-(naphthalen-1-ylethynyl)-3-deaza-2′-deoxyguanosine discriminates cytidine via the DNA minor groove. Issue 37 (7th September 2017)

• Record Type: Journal Article
• Authors: Suzuki, Azusa; Yanagi, Masaki; Takeda, Takuya; Hudson, Robert H. E.; Saito, Yoshio
• Is Part Of: Organic & biomolecular chemistry. Volume 15:Issue 37(2017)
• Page Start: 7853
• Page End: 7859
• Publication Date: 2017-09-07
• Subjects: Chemistry, Organic -- Periodicals; Bioorganic chemistry -- Periodicals; Chemistry, Physical organic -- Periodicals; 547
• DOI: 10.1039/c7ob01931a
• Languages: English
• ISSNs: 1477-0520

Abstract

Abstract : A newly synthesized fluorescent nucleoside 3nz G discriminates cytidine in target DNA strands by a distinct change in its emission wavelength. Abstract : A new environmentally responsive fluorescent nucleoside, 3-(naphthalen-1-ylethynyl)-3-deaza-2′-deoxyguanosine ( 3nz G ), has been synthesized. The nucleoside, 3nz G, exhibited solvatochromic properties and when introduced into ODN probes it was able to recognize 2′-deoxycytidine in target strands by a distinct change in its emission wavelength through probing microenvironmental changes in the DNA minor groove. Thus, 3nz G has the potential for use as a fluorescent probe molecule for micro-structural studies of nucleic acids including the detection of single-base alterations in target DNA sequences.