Site-selective tagging of proteins by pnictogen-mediated self-assembly. Issue 79 (11th September 2017)

• Record Type: Journal Article
• Authors: Nitsche, Christoph; Mahawaththa, Mithun C.; Becker, Walter; Huber, Thomas; Otting, Gottfried
• Is Part Of: Chemical communications. Volume 53:Issue 79(2017)
• Page Start: 10894
• Page End: 10897
• Publication Date: 2017-09-11
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/c7cc06155b
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : Trivalent pnictogens (Pn) enable the selective self-assembly between an engineered di-cysteine motif in a protein and a thiol-containing lanthanide (Ln) probe. Abstract : Site-selective chemical protein modification is achieved by self-assembly of a specific di-cysteine motif, trivalent pnictogens (As, Sb or Bi) and an aromatic mercaptomethyl-based probe. The strategy is demonstrated with a quaternary complex involving Zika virus protease and a lanthanide ion, enabling paramagnetic nuclear magnetic resonance spectroscopy and luminescence measurements.