DNA methylation-mediated silencing of FLT1 in parthenogenetic porcine placentas. (October 2017)

• Record Type: Journal Article
• Authors: Song, Yuning; Liu, Zhiquan; Han, Yang; Gao, Wei; Hao, Jindong; Zhong, Xiaowei; Wang, Dongxu; Li, Zhanjun
• Is Part Of: Placenta. Volume 58(2017:Oct.)
• Page Start: 86
• Page End: 89
• Publication Date: 2017-10
• Subjects: VEGF-A -- PIGF -- FLT1 -- KDR -- DNA methylation -- Parthenogenetic; Placenta -- Periodicals; Reproduction -- Periodicals; Placenta -- Periodicals; Placenta -- Périodiques; Reproduction -- Périodiques; 612.63
• DOI: 10.1016/j.placenta.2017.08.074
• Languages: English
• ISSNs: 0143-4004

Abstract

Abstract: Studies show that perturbed expression of vascular endothelial growth factor ( VEGF ) family genes is related to abnormal development of parthenogenetic (PA) placenta. In this study, the methylation status of VEGF family genes were compared between PA and normal placentas using bisulfite sequencing PCR (BSP). Results showed no significant difference in the methylation of VEGF-A differentially methylated region (DMR), placental growth factor ( PIGF ) DMR, and kinase insert domain receptor ( KDR ) DMR, whereas FMS-like tyrosine kinase 1 ( FLT1 ) DMR was hypermethylated in PA placentas. These results suggested that abnormal methylation of FLT1 DMR might trigger the developmental failure of porcine PA placentas. Highlights: VEGF-A, PIGF and KDR were hypomethylated in porcine PA placentas. FLT1 were hypermethylated in PA placentas. Abnormal methylation status of FLT1 causes development defect of placentas.