A Non‐Perilous Coumarin‐Based Ratiometric Probe for ′In Vitro′ Detection of Cu through Cell Imaging Technique. Issue 27 (21st September 2017)

• Record Type: Journal Article
• Authors: Kumari, Chanda; Sain, Dibyendu; Kumar, Ashish; Nayek, Hari Pada; Debnath, Sushanta; Saha, Partha; Dey, Swapan
• Is Part Of: ChemistrySelect. Volume 2:Issue 27(2017)
• Page Start: 8270
• Page End: 8277
• Publication Date: 2017-09-21
• Subjects: Cell imaging -- Copper -- Coumarin -- Fluorescent sensor -- In vitro analysis -- Rhodamine -- Strip test; Chemistry -- Periodicals; 540.5
• DOI: 10.1002/slct.201701482
• Languages: English
• ISSNs: 2365-6549

Abstract

Abstract: A novel coumarin based fluorescent turn on probe (R1 ) was designed and synthesized for 'on the spot' detection of biologically importantCu 2+ . The actual three dimensional geometry of the probe was confirmed by single crystal X‐ray analysis.R1 showed high sensitivity and selectivity towardsCu 2+ without any intervention of other metal ions. Rapid naked eye detection ofCu 2+ through colour change increased the superiority of the probe for practical use. Absorption and emission spectra proved the ratiometric detection ability of the probe towardsCu 2+ . Long wavelength visual fluorescence emission of the complex was utilized for intracellular detection ofCu 2+ in living cells. Abstract : A coumarin appended rhodamine probe (R1) was synthesized for the selective detection of Cu 2+ . The three dimensional structure of probe R1 was confirmed by solid state structure of R1. Probe R1 also showed its real time application to recognise Cu 2+ in live HeLa cell.