Toxin production, growth kinetics and molecular characterization of Ostreopsis cf. ovata isolated from Todos os Santos Bay, tropical southwestern Atlantic. (November 2017)

• Record Type: Journal Article
• Authors: Mendes, Maria Cristina de Q.; Nunes, José Marcos C.; Menezes, Mariângela; Fraga, Santiago; Rodríguez, Francisco; Vázquez, José A.; Blanco, Juan; Franco, José M.; Riobó, Pilar
• Is Part Of: Toxicon. Volume 138(2017)
• Page Start: 18
• Page End: 30
• Publication Date: 2017-11
• Subjects: Growth -- N -- P -- Ovatoxins -- Hemolytic assay -- Phylogenetic analysis; Toxins -- Periodicals; Venom -- Periodicals; 615.9
• DOI: 10.1016/j.toxicon.2017.08.007
• Languages: English
• ISSNs: 0041-0101

Abstract

Abstract: The toxin profile and hemolytic activity of a strain of Ostreopsis cf. ovata (UFBA013) isolated from Todos os Santos Bay (northeastern Brazil) were evaluated under different levels of N and P. Phylogenetic analyses based on ITS rDNA region (ITS1-5.8S-ITS2) placed UFBA013 within the Atlantic/Mediterranean/Pacific clade of O . cf. ovata . Growth experiments were conducted in f/2 medium modified by adding N and P (P: 0–36 μM; N: 0–882 μM). The growth kinetics was adequately described by logistic equations. The best growth (highest G m ) was recorded under levels of N/P = 0/18, 129/5 and 441/36, while one of the lowest G m was obtained under P-depletion. The maximum and specific maximum growth rates (as v m; cells mL −1 d −1 and μ m ; d −1 ) were achieved with N limitation (N/P = 441/36) and P-limitation/depletion (753/5.3 and 441/0) and are the highest values reported in the literature, most similar to isolates from Pacific and Mediterranean areas. The control experiment (N/P = 441/18) also yielded similar values to those from some Mediterranean isolates, but higher than formerly reported for Brazilian isolates. In all conditions assayed, no palytoxin (PLTX) was detected. The ovatoxins (OVTXs) a, b, c, d and e did not show significant differences in cell quota between exponential and stationary phases. A significant relationship was detected between OVTXs concentration and hemolytic activity. Highlights: Nutrient influences on cell growth and toxin production of O. cf. ovata. ITS rDNA phylogeny placed tropical O. cf. ovata in Atlantic/Med/Pacific clade. LC-HRMS analyses revealed the presence of OVXTs - a to -e and absence of PLTX. Growth rates affected by nutrient conditions but no clear trends in toxins.