The high‐quality genome of Brassica napus cultivar 'ZS11' reveals the introgression history in semi‐winter morphotype. (9th October 2017)

• Record Type: Journal Article
• Authors: Sun, Fengming; Fan, Guangyi; Hu, Qiong; Zhou, Yongming; Guan, Mei; Tong, Chaobo; Li, Jiana; Du, Dezhi; Qi, Cunkou; Jiang, Liangcai; Liu, Weiqing; Huang, Shunmou; Chen, Wenbin; Yu, Jingyin; Mei, Desheng; Meng, Jinling; Zeng, Peng; Shi, Jiaqin; Liu, Kede; Wang, Xi; Wang, Xinfa; Long, Yan; Liang, Xinming; Hu, Zhiyong; Huang, Guodong; Dong, Caihua; Zhang, He; Li, Jun; Zhang, Yaolei; Li, Liangwei; Shi, Chengcheng; Wang, Jiahao; Lee, Simon Ming‐Yuen; Guan, Chunyun; Xu, Xun; Liu, Shengyi; Liu, Xin; Chalhoub, Boulos; Hua, Wei; Wang, Hanzhong;
• Is Part Of: Plant journal. Volume 92:Number 3(2017)
• Page Start: 452
• Page End: 468
• Publication Date: 2017-10-09
• Subjects: Brassica napus -- allotetraploid -- genome -- homoeolog -- introgression -- next‐generation sequencing technologies -- polyploid -- subgenome -- ZS11; Plant molecular biology -- Periodicals; Plant cells and tissues -- Periodicals; Botany -- Periodicals; 580
• DOI: 10.1111/tpj.13669
• Languages: English
• ISSNs: 0960-7412

Abstract

Summary: Allotetraploid oilseed rape ( Brassica napus L.) is an agriculturally important crop. Cultivation and breeding of B . napus by humans has resulted in numerous genetically diverse morphotypes with optimized agronomic traits and ecophysiological adaptation. To further understand the genetic basis of diversification and adaptation, we report a draft genome of an Asian semi‐winter oilseed rape cultivar 'ZS11' and its comprehensive genomic comparison with the genomes of the winter‐type cultivar 'Darmor‐ bzh ' as well as two progenitors. The integrated BAC‐to‐BAC and whole‐genome shotgun sequencing strategies were effective in the assembly of repetitive regions (especially young long terminal repeats) and resulted in a high‐quality genome assembly of B . napus 'ZS11'. Within a short evolutionary period (~6700 years ago), semi‐winter‐type 'ZS11' and the winter‐type 'Darmor‐ bzh ' maintained highly genomic collinearity. Even so, certain genetic differences were also detected in two morphotypes. Relative to 'Darmor‐ bzh ', both two subgenomes of 'ZS11' are closely related to its progenitors, and the 'ZS11' genome harbored several specific segmental homoeologous exchanges (HEs). Furthermore, the semi‐winter‐type 'ZS11' underwent potential genomic introgressions with B . rapa (Ar ). Some of these genetic differences were associated with key agronomic traits. A key gene of A03. FLC3 regulating vernalization‐responsive flowering time in 'ZS11' was first experienced HE, and then underwent genomic introgression event with Ar, which potentially has led to genetic differences in controlling vernalization in the semi‐winter types. Our observations improved our understanding of the genetic diversity of different B . napus morphotypes and the cultivation history of semi‐winter oilseed rape in Asia. Significance Statement: We generated a high‐quality reference genome sequence of semi‐winter Brassica napus . Our BAC‐to‐BAC strategy was effective in the assembly of repetitive regions. The main phenotypic difference between semi‐winter and winter B. napus morphotypes is the vernalization requirement. Here, we found that a key gene controlling the vernalization‐responsive flowering time was altered in the semi‐winter type 'ZS11' through both homoeologous exchanges and genomic introgression with B. rapa .