Flavonoid Composition of Tarocco (Citrus sinensis L. Osbeck) Clone "Lempso" and Fast Antioxidant Activity Screening by DPPH‐UHPLC‐PDA‐IT‐TOF. Issue 6 (7th July 2017)

• Record Type: Journal Article
• Authors: Sommella, Eduardo; Pagano, Francesco; Pepe, Giacomo; Ostacolo, Carmine; Manfra, Michele; Chieppa, Marcello; Di Sanzo, Rosa; Carabetta, Sonia; Campiglia, Pietro; Russo, Mariateresa
• Is Part Of: Phytochemical analysis. Volume 28:Issue 6(2017:Nov.)
• Page Start: 521
• Page End: 528
• Publication Date: 2017-07-07
• Subjects: Tarocco Lempso -- anthocyanins -- flavonoids -- antioxidant -- DPPH‐UHPLC‐PDA; Plants -- Analysis -- Periodicals; Plants -- chemistry -- Periodicals; 572.2
• DOI: 10.1002/pca.2701
• Languages: English
• ISSNs: 0958-0344

Abstract

Abstract: Introduction: Clonal selection and hybridisation are valid strategies to obtain fruits with enhanced sensorial and nutraceutical properties. Within Citrus sinensis varieties, Tarocco clone "Lempso" is a typical product of the Calabria region (Italy) characterised by its red pulp. This is the first report concerning its accurate profiling. Objective: To characterise in detail the flavonoid composition of Lempso clone and to compare its antioxidant potential with other Citrus varieties by a fast screening method. Methodology: Extracts were subjected to solid phase extraction and the qualitative/quantitative profile was elucidated through ultra‐high performance liquid chromatography (UHPLC) coupled to photodiode array (PDA) and ion trap time‐of‐flight (IT‐TOF) mass spectrometry detection, and compared to both Cleopatra mandarin ( Citrus reticulata ) and blood orange ( Citrus sinensis (L.) Osbeck) Sanguinello varieties. The antioxidant activity was assessed by pre‐column 2, 2′‐diphenyl‐1‐picrylhydrazyl (DPPH) reaction coupled to UHPLC‐PDA. Results: Lempso is characterised by flavonoids (17) and anthocyanins (8). Flavanones content (Hesperidin: 57.19 ± 0.49, Vicenin‐2: 4.59 ± 0.03, Narirutin: 5.78 ± 0.13 mg/100 mL) was considerably higher than Cleopatra and Sanguinello varieties. The developed DPPH‐UHPLC‐PDA method provides information regarding the single contributions to antioxidant activity, highlighting how Ferulic acid, Quercetin and Cyanidin derivatives possess considerable radical scavenging activity (> 50%). The total antioxidant activity was also evaluated and compared with positive controls, showing higher scavenging activity than Cleopatra and Sanguinello (IC50 : 333.76 ± 10.81 μg/mL vs. 452.62 ± 10.81 and 568.39 ± 26.98 μg/mL, respectively). Conclusion: These data evidence the nutraceutical potential of Lempso variety, which could be an ingredient for functional beverages. Copyright © 2017 John Wiley & Sons, Ltd. Abstract : The flavonoid pattern of a typical Citrus sinensis variety, an original product of the Calabria region, namely Tarocco Lempso, has been investigated thoroughly. The qualitative/quantitative profiling was performed using a UHPLC‐PDA‐IT‐TOF platform. Moreover, a precolumn DPPH assay coupled to UHPLC‐PDA was developed as a fast antioxidant screening method. The entire protocol takes only 50 min per sample, comprising of reaction and analysis. The developed method highlighted higher amount of flavanones and a better antioxidant activity of Lempso clone, in comparison with other Citrus varieties.