Amino acid functionalized zinc oxide nanostructures for cytotoxicity effect and hemolytic behavior: Theoretical and experimental studies. (15th November 2017)

• Record Type: Journal Article
• Authors: Singh, Satvinder; Singh, Baljinder; Sharma, Prateek; Mittal, Anu; Kumar, Sanjeev; Saini, G.S.S.; Tripathi, S.K.; Singh, Gurinder; Kaura, Aman
• Is Part Of: Materials & design. Volume 134(2017)
• Page Start: 10
• Page End: 22
• Publication Date: 2017-11-15
• Subjects: Zinc oxide nanoparticles -- Binding energy -- Cytotoxicity -- Hemolysis -- Partial density of states; Materials -- Periodicals; Engineering design -- Periodicals; Matériaux -- Périodiques; Conception technique -- Périodiques; Electronic journals; 620.11
• DOI: 10.1016/j.matdes.2017.08.020
• Languages: English
• ISSNs: 0264-1275

Abstract

Abstract: Blending of theoretical and experimental approach, provide an important strategy in designing the nanostructure at a microscopic level and helps in predicting the response of synthesized material towards inhibition of the growth of breast cancer cell. In this work, ab initio calculations using super cell approach are performed for three different amino acids (AAs)-Histidine (His), Arginine (Arg) and Aspartic acid (Asp) coated Zinc oxide (ZnO) nanostructures to explain the growth mechanism of nanoparticles (NPs) of different shapes. Based on the first principles calculations, we reveal that ZnO-AA (Arg and Asp) NPs results in rod like and ZnO-His NPs lead to tablet like configuration. Similar morphologies are fabricated using AAs through synthetic route. The effect of concentration ratio of reactants and pH has been studied. As synthesized samples, are characterized by using Transmission Electron Microscopy (TEM), X-ray diffraction (XRD), Fourier Transform Infrared (FTIR) and UV–Vis spectroscopy techniques. Based on the results, a plausible mechanism of formation of nanostructures has been proposed. The nanostructures with rod like morphology are found to be biocompatible with normal red blood cells and show cytotoxic effect as evaluated from hemolysis and cytotoxicity assays on breast (MCF-7, T47D, MDA-MB-231) & prostate cancer (PC-3) cell lines. Graphical abstract: Highlights: First principles method provides design rule in fabricating Zinc oxide nanostructures of specific shape. Based on affinity of different amino acids with Zinc Oxide nanostructures, we predict rod and tablet morphologies. These predictions have been proved experimentally by synthesizing the nanostructures of the desired shape. Synthesized material of rod like morphology shows more cytotoxic effect on cancer cells and is more biocompatible.