Emergence of a colistin-resistant Escherichia coli clinical isolate harboring mcr-1 in Japan. (October 2017)

• Record Type: Journal Article
• Authors: Tada, Tatsuya; Uechi, Kohei; Nakasone, Isamu; Shimada, Kayo; Nakamatsu, Masashi; Kirikae, Teruo; Fujita, Jiro
• Is Part Of: International journal of infectious diseases. Volume 63(2017:Oct.)
• Page Start: 21
• Page End: 22
• Publication Date: 2017-10
• Subjects: Communicable diseases -- Periodicals; Communicable Diseases -- Periodicals; Communicable diseases; Periodicals; Electronic journals; 616.9
• DOI: 10.1016/j.ijid.2017.07.023
• Languages: English
• ISSNs: 1201-9712

Abstract

Highlights: A colistin-resistant Escherichia coli isolate harboring mcr-1 was isolated in a university hospital in Japan. Pulsed-field gel electrophoresis and Southern hybridization revealed that mcr-1 was located on a 130-kb plasmid. The genomic environment surrounding mcr-1 had 99.99% identity with those in Citrobacter braakii plasmid from Bolivia and Salmonella enterica serovar Typhimurium plasmid from China. This is the first report of a clinical isolate harboring mcr-1 in Japan. Abstract: The mcr-1 is a gene encoding a phosphoethanolamine transferase, which confers resistance to colistin by transferring phosphoethanolamine to lipid A. We describe here the emergence of a colistin-resistant Escherichia coli clinical isolate harboring plasmid-mediated mcr-1 in Japan. The isolate belonged to ST5702 and is suspected to come from livestock and transmitted to human. This is the first report of a clinical isolate harboring mcr-1 in Japan.