Active subsite properties, subsite residues and targeting to lysosomes or midgut lumen of cathepsins L from the beetle Tenebrio molitor. (October 2017)

• Record Type: Journal Article
• Authors: Damasceno, Ticiane F.; Dias, Renata O.; de Oliveira, Juliana R.; Salinas, Roberto K.; Juliano, Maria A.; Ferreira, Clelia; Terra, Walter R.
• Is Part Of: Insect biochemistry and molecular biology. Volume 89(2017)
• Page Start: 17
• Page End: 30
• Publication Date: 2017-10
• Subjects: Cathepsin L-like peptidase -- Substrate specificity -- Subsite role -- Subsite hydrophobicity -- Phosphotransferase -- Cathepsin L activation -- Tenebrio molitor; Abz ortho-aminobenzoic acid -- DTT dithithreitol -- E64 trans-epooxysuccinyl-L-leucylamido-(4-guanidino)butane -- EDDnp N-(2, 4-dinitrophenyl)ethylenediamine -- FRET fluorescence resonance energy transfer -- LERP lysosomal enzyme receptor protein -- M6P mannose 6–phosphate -- MPR mannose-6-phosphate receptor -- phosphotransferase N-acetylglucosaminidase-1-phosphate transferase -- uncovering enzyme N-acetylglucosamine-1-phosphodiester α-N-acetylglucosaminidase -- TGN trans-Golgi network -- Z-FR-MCA benzyloxycarbonyl-L-phenylalanyl-L-arginine 4-methylcoumarin-7-amide; Insect biochemistry -- Periodicals; Insects -- Physiology -- Periodicals; Insects -- Molecular aspects -- Periodicals; Biochemistry -- Periodicals; Insectes -- Biochimie -- Périodiques; Insectes -- Composition -- Périodiques; Insectes -- Physiologie -- Périodiques; Insectes -- Aspect moléculaire -- Périodiques; Biochimie -- Périodiques; Biochemistry; Insect biochemistry; Insects -- Molecular aspects; Insects -- Physiology; Periodicals; 572.8157
• DOI: 10.1016/j.ibmb.2017.08.004
• Languages: English
• ISSNs: 0965-1748

Abstract

Abstract: Cathepsins L are the major digestive peptidases in the beetle Tenebrio molitor . Two digestive cathepsins L (TmCAL2 and TmCAL3) from it had their 3D structures solved. The aim of this paper was to study in details TmCAL3 specificity and properties and relate them to its 3D structure. Recombinant TmCAL3 was assayed with 64 oligopeptides with different amino acid replacements in positions P2, P1, P1′ and P2'. Results showed that TmCAL3 S2 specificity differs from the human enzyme and that its specificities also explain why on autoactivation two propeptide residues remain in the enzyme. Data on free energy of binding and of activation showed that S1 and S2′ are mainly involved in substrate binding, S1′ acts in substrate binding and catalysis, whereas S2 is implied mainly in catalysis. Enzyme subsite residues were identified by docking with the same oligopeptide used for kinetics. The subsite hydrophobicities were calculated from the efficiency of hydrolysis of different amino acid replacements in the peptide and from docking data. The results were closer for S1 and S2′ than for S1' and S2, indicating that the residue subsites that were more involved in transition state binding are different from those binding the substrate seen in docking. Besides TmCAL1-3, there are nine other cathepsins L, most of them more expressed at midgut. They are supposed to be directed to lysosomes by a Drosophila-like Lerp receptor and/or motifs in their prodomains. The mannose 6-phosphate lysosomal sorting machinery is absent from T. molitor transcriptome. Cathepsin L direction to midgut contents seems to depend on overexpression. Graphical abstract: Highlights: There are cathepsin Ls (TmCALs) in T. molitor midgut, which may be directed to midgut contents depending on overexpression. The major TmCAL3 was assayed with 64 oligopeptides with different amino acid replacements to study its specificity. Enzyme subsite residues were predicted by docking into the 3D structure of TmCAL3 with the oligopeptide used for kinetics.