Production of chitin and chitosan from the exoskeleton of adult two‐spotted field crickets (Gryllus bimaculatus). Issue 5 (27th September 2017)

• Record Type: Journal Article
• Authors: Kim, Min‐Woo; Song, Yong‐Su; Han, Yeon Soo; Jo, Yong Hun; Choi, Myung Hyo; Park, Young‐Kyu; Kang, Seung Ho; Kim, Sun‐Am; Choi, Changyong; Jung, Woo‐Jin
• Is Part Of: Entomological research. Volume 47:Issue 5(2017:Sep.)
• Page Start: 279
• Page End: 285
• Publication Date: 2017-09-27
• Subjects: adult exoskeleton -- chitin -- chitosan -- Cricket (Gryllus bimaculatus); Insects -- Korea -- Periodicals; Entomology -- Periodicals; 595.709519
• DOI: 10.1111/1748-5967.12239
• Languages: English
• ISSNs: 1738-2297

Abstract

Abstract: Chitin and chitosan were extracted from all specimens of Type I and II two‐spotted field crickets ( Gryllus bimaculatus ) following chemical treatment with an acid and alkali. For chitin extraction, 2 N HCl and 1.25 N NaOH solutions were used to achieve demineralization and deproteinization, respectively. For chitosan extraction, 50 % NaOH ( w / v ) and 50 % NaOH ( w /w) solutions were used to achieve deacetylation. Chitosan yielded from adult exoskeletons of G. bimaculatus in Test A of Type I was 1.76 and 8.40 % on a fresh weight (FW) and dry weight (DW) basis, respectively, after treatment with 50 % NaOH ( w / v ) at 95°C for 3 h. Furthermore, the chitosan yielded in Test D of Type II was 1.79 and 7.06 % on FW and DW basis, respectively, after treatment with 50 % NaOH ( w /w) at 105°C for 3 h. The average yield of chitin and chitosan was 2.42 and 1.65 % on a FW basis, and 10.91 and 7.50 % on DW basis, respectively. The deacetylation (%) of chitosan extracted from adult exoskeletons in Tests A, B, C1, C2, D1, and D2 were 81.2 %, 14.5 %, 19.6 %, 90.7 %, 17.1 %, and 95.5 %, respectively. The viscosities of the chitosans extracted from adult exoskeletons in Tests A, C2, and D2 were 32.0, 21.6, and 62.4 cP (centi Poise), respectively. The molecular weight of chitosan from adult exoskeletons of G. bimaculatus was 308.3 kDa. Our results indicate that adult exoskeletons of G. bimaculatus could be used as a source of chitin and chitosan for use as functional additives in industrial animal feeds.