Analysis of the BRAFV600E mutation in 19 cases of Langerhans cell histiocytosis in Japan. Issue 3 (4th April 2016)

• Record Type: Journal Article
• Authors: Sasaki, Yuya; Guo, Ying; Arakawa, Fumiko; Miyoshi, Hiroaki; Yoshida, Noriaki; Koga, Yuhki; Nakashima, Kazutaka; Kurita, Daisuke; Niino, Daisuke; Seto, Masao; Ohshima, Koichi
• Is Part Of: Hematological oncology. Volume 35:Issue 3(2017:Sep.)
• Page Start: 329
• Page End: 334
• Publication Date: 2016-04-04
• Subjects: Langerhans cell histiocytosis -- BRAFV600E -- Japan; Hematological oncology -- Periodicals; Hematology; Medical Oncology; 616.99418005
• DOI: 10.1002/hon.2293
• Languages: English
• ISSNs: 0278-0232

Abstract

Abstract: Langerhans cell histiocytosis (LCH) is a rare disease characterized by clonal proliferation of CD1a‐ and CD207 (langerin)‐positive dendritic cells. Mutated BRAF ( p.V600E ) is observed in histiocyte‐related diseases and dendritic cell‐related diseases, including LCH. BRAFV600E is observed in some LCH cases and is thought to be involved in maintaining MAPK activation. We retrospectively analyzed BRAFV600E in 19 patients diagnosed with LCH. In our study, direct sequencing for exon 15, a mutation hotspot, demonstrated that 4 out of the 19 patients (21%) harbored a GTG > GAG (valine > glutamic acid) base substitution, which encodes BRAFV600E. The clinical impact of BRAFV600E in such diseases is unclear. The frequency of BRAFV600E in our LCH patients from Japan was lower than that reported in the United States and in Germany. However, reports from Asia tend to show a lower rate of the BRAFV600E mutation. These results imply the possibility of different genetic backgrounds in the pathogenesis of LCH across various ethnicities. We also performed an immunohistochemical analysis to detect BRAFV600E using the mutation‐specific monoclonal antibody. However, immunohistochemical analysis failed to detect any mutated protein in any of the 4 BRAFV600E ‐positive cases. This implies that at present, BRAFV600E should be assessed by direct sequencing. Copyright © 2016 John Wiley & Sons, Ltd.