High throughput in vitro and in vivo screening of inland waters of Southern California. Issue 9 (14th June 2017)

• Record Type: Journal Article
• Authors: Mehinto, A. C.; VanDervort, D. R.; Lao, W.; He, G.; Denison, M. S.; Vliet, S. M.; Volz, D. C.; Mazor, R. D.; Maruya, K. A.
• Is Part Of: Environmental science. Volume 19:Issue 9(2017)
• Page Start: 1142
• Page End: 1149
• Publication Date: 2017-06-14
• Subjects: Environmental monitoring -- Periodicals; Biological monitoring -- Periodicals; Environmental chemistry -- Periodicals; 363.7363
• DOI: 10.1039/c7em00170c
• Languages: English
• ISSNs: 2050-7887

Abstract

Abstract : High throughput in vitro and in vivo screening assays were combined to evaluate contaminant impacts on the health of freshwater streams. Abstract : The impact of unmonitored contaminants, also known as contaminants of emerging concern (CECs), on freshwater streams remains largely uncharacterized. Water samples from 31 streams representing urban, agricultural and undeveloped ( i.e., open space) land use in Southern California (USA) were analyzed for in vitro and in vivo bioactivity. The extent and magnitude of bioactivity screened using endocrine-responsive cell bioassays and a fish embryo screening assay were low. In contrast, a wider gradient of responses for the aryl hydrocarbon receptor (AhR) assay was observed, which was negatively correlated with a measure of benthic community structure. Both aromatic and non-aromatic CECs were tentatively identified in these samples, but polycyclic aromatic hydrocarbons (PAHs), known AhR agonists in urban environments, were not present at detectable levels. These results suggest that a combination of in vitro and in vivo show potential as screening techniques for biological condition in situ, but that more advanced, comprehensive analytical methods are needed to identify bioactive contaminants.