A mutation-resistant deoxyribozyme OR gate for highly selective detection of viral nucleic acids. Issue 76 (13th September 2017)

• Record Type: Journal Article
• Authors: Kamar, Ola; Sun, Sin-Cih; Lin, Chieh-Hua; Chung, Wan-Yu; Lee, Min-Shi; Liao, Yu-Chieh; Kolpashchikov, Dmitry M.; Chuang, Min-Chieh
• Is Part Of: Chemical communications. Volume 53:Issue 76(2017)
• Page Start: 10592
• Page End: 10595
• Publication Date: 2017-09-13
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/c7cc05576e
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : A set of deoxyribozyme probes was arranged in an OR gate to fluorescently report ∼90% of enterovirus 71 strains and distinguish them from coxsackievirus. Abstract : Highly selective probes hybridize only to fully complementary DNA or RNA sequences and, therefore, often fail to recognize mutated viral genomes. Here we designed a probe that possesses two seemingly incompatible properties: it tolerates some point mutations in genome, while it remains selective towards others. An OR deoxyribozyme logic gate was designed to fluorescently report the sequences of enterovirus 71 (EV71) covering ∼90% of all known EV71 strains. Importantly, sequences of closely related coxsackieviruses that differed by single nucleotides were reliably differentiated in 7 out of 8 cases.