Target-induced cyclic DNAzyme formation for colorimetric and chemiluminescence imaging assay of protein biomarkers. Issue 19 (7th September 2017)

• Record Type: Journal Article
• Authors: Yang, Kaili; Huo, Min; Guo, Yuehua; Yang, Yizhuo; Wu, Jie; Ding, Lin; Ju, Huangxian
• Is Part Of: Analyst. Volume 142:Issue 19(2017)
• Page Start: 3740
• Page End: 3746
• Publication Date: 2017-09-07
• Subjects: Chemistry, Analytic -- Periodicals; 543
• DOI: 10.1039/c7an00413c
• Languages: English
• ISSNs: 0003-2654

Abstract

Abstract : A target-induced cyclic strategy for DNAzyme formation was developed for simple and sensitive colorimetric and chemiluminescence detection of protein biomarkers. Abstract : A target-induced cyclic strategy for DNAzyme formation was proposed to achieve simple, sensitive and universal detection of protein biomarkers with convenient colorimetric or chemiluminescence imaging readout. In the assay, the target protein was recognized by a pair of DNA-labeled antibodies (Ab1-DNA1 and Ab2-DNA2) to form a proximate complex, which could hybridize with the conjugate DNA3/DNA4 to release the guanine-rich DNA4 and thus formed G-quadruplex/hemin horseradish peroxidase-mimicking DNAzyme. The process could be further recycled with Exonuclease III by cleaving DNA3 to free the proximate complex, resulting in the cyclic formation of DNAzyme. The G-quadruplex/hemin DNAzyme could catalyze the H2 O2 -mediated oxidation of 3, 3, 5, 5-tetramethylbenzidine to produce the color change from colorless to blue or enhance the chemiluminescence of a luminol–H2 O2 system. Thus the signal could be read out with the naked eye, and by colorimetry and chemiluminescence imaging. Using a carcinoembryonic antigen as a model target, the proposed assay showed a detection range of 4 orders of magnitude along with detection limits of 170 and 16 pg mL −1 for colorimetric and chemiluminescence imaging assays respectively. This assay had the advantages of easy operation, sensitive detection, target flexibility and diversified signal readout, providing a great opportunity for commercial application.