A ratiometric fluorescent probe for imaging and quantifying anti-apoptotic effects of GSH under temperature stress. Issue 10 (24th August 2017)

• Record Type: Journal Article
• Authors: Han, Xiaoyue; Song, Xinyu; Yu, Fabiao; Chen, Lingxin
• Is Part Of: Chemical science. Volume 8:Issue 10(2017)
• Page Start: 6991
• Page End: 7002
• Publication Date: 2017-08-24
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/c7sc02888a
• Languages: English
• ISSNs: 2041-6520

Abstract

Abstract : A ratiometric fluorescent probe for imaging and quantifying concentration fluctuations and anti-apoptotic effects of GSH under hypothermia and hyperthermia in HepG2 and HepG2/DDP xenografts. Abstract : Hypothermia and hyperthermia are cell stressed states resulting from environmental temperature changes, which can abnormally decrease intracellular glutathione (GSH) concentrations and induce apoptosis. As the most abundant intracellular non-protein biothiol, GSH can protect cells from apoptosis. Considering the important roles of GSH in the anti-apoptotic process in cells and in vivo, we strive to develop a powerful chemical tool for the direct detection of GSH concentration changes under temperature stress. Herein, we report a ratiometric fluorescent probe (CyO-Dise ) based on a selenium–sulfur exchange reaction for the qualitative and quantitative detection of GSH concentration fluctuations in cells and in vivo . The probe has been successfully used to assess the changes of GSH levels in HepG2 and HL-7702 cells using the stimulations of hypothermia and hyperthermia. In terms of the anti-apoptotic effect of GSH under hypothermic and hyperthermic conditions, human normal liver HL-7702 cells have stronger abilities to fight against temperature stress than human liver carcinoma HepG2 cells. Hypothermia and hyperthermia can also improve the drug resistance of cis -dichlorodiamineplatinum(ii ) (DDP)-resistant HepG2/DDP cells. TheCyO-Dise probe has been employed to image GSH concentration changes in HepG2 and HepG2/DDP xenografts on nude mice. With the adjuvant therapy effects of hypothermia and hyperthermia, the chemotherapy drug DDP exhibits good ability for the treatment of HepG2 and HepG2/DDP xenografts. The above applications make our probe a potential new candidate for the accurate diagnosis of cancer and efficacy evaluation of treatment.