Detection of Mycobacterium tuberculosis from paraffin-embedded tissues by GeneXpert MTB/RIF. (September 2017)

• Record Type: Journal Article
• Authors: Rindi, Laura; Alì, Greta; Fabiani, Barbara; Fontanini, Gabriella; Garzelli, Carlo
• Is Part Of: Tuberculosis. Volume 106(2017)
• Page Start: 53
• Page End: 55
• Publication Date: 2017-09
• Subjects: 616.995
• DOI: 10.1016/j.tube.2017.06.005
• Languages: English
• ISSNs: 1472-9792

Abstract

Abstract: GeneXpert MTB/RIF (Xpert) assay, a rapid and automated system based on real-time PCR and molecular beacon technology, proved to be a sensitive and specific tool capable of detecting Mycobacterium tuberculosis and rifampin resistance in clinical specimens. In this study we provide a Xpert-dedicated successful protocol for processing paraffin-embedded tissue and assess the feasibility of the Xpert assay-based tuberculosis (TB) diagnosis on these specimens, thus proving the Xpert assay as a valuable TB diagnostic tool in supporting conventional histopathological methods.