Dietary administration of tetrodotoxin and its putative biosynthetic intermediates to the captive-reared non-toxic Japanese fire-bellied newt, Cynops pyrrhogaster. (October 2017)

• Record Type: Journal Article
• Authors: Kudo, Yuta; Chiba, Chikafumi; Konoki, Keiichi; Cho, Yuko; Yotsu-Yamashita, Mari
• Is Part Of: Toxicon. Volume 137(2017)
• Page Start: 78
• Page End: 82
• Publication Date: 2017-10
• Subjects: Cynops pyrrhogaster -- LC-MS -- Newt -- Tetrodotoxin -- Toxin administration; Toxins -- Periodicals; Venom -- Periodicals; 615.9
• DOI: 10.1016/j.toxicon.2017.07.016
• Languages: English
• ISSNs: 0041-0101

Abstract

Abstract: The origin of tetrodotoxin (TTX) in amphibians has long been disputed. In this study, TTX and its putative biosynthetic intermediates or shunt compounds (4, 9-anhydro-10-hemiketal-5-deoxyTTX and Cep-242) were dietary administered to the captive-reared, non-toxic Japanese fire-bellied newt, Cynops pyrrhogaster . After 4 weeks, the ingested compounds were detected mainly in the newt body using liquid chromatography-mass spectrometry (LC-MS), while these compounds were not converted into other TTX analogues in newts. Highlights: Tetrodotoxin (TTX) and its related compounds were dietary administered to the newt. TTX was detected mainly in the body of the newt 4 weeks after administration. TTX and its related compounds in the tested newt were hardly secreted for 4 weeks. Administered compounds were not converted into other analogues in the newt.