Comparative proteomic analysis of Ganoderma species during in vitro interaction with oil palm root. (August 2017)

• Record Type: Journal Article
• Authors: Al-Obaidi, Jameel R.; Hussin, Siti Nahdatul Isnaini Said; Saidi, Noor Baity; Rahmad, Norasfaliza; Idris, Abu Seman
• Is Part Of: Physiological and molecular plant pathology. Volume 99(2017:Jul.)
• Page Start: 16
• Page End: 24
• Publication Date: 2017-08
• Subjects: Ganoderma boninense -- Ganoderma tornatum -- Fungal proteomics -- Fungal virulence; Plant diseases -- Periodicals; Diseased plants -- Physiology -- Periodicals; Phytopathogenic microorganisms -- Host plants -- Periodicals; 632
• DOI: 10.1016/j.pmpp.2017.02.001
• Languages: English
• ISSNs: 0885-5765

Abstract

Abstract: Basal stem rot disease caused by Ganoderma spp. is still considered a large threat to oil palm production in many countries, especially in Malaysia, which contributes to approximately 45% of the world's palm oil exports. In the last 10 years, studies on the oil palm- Ganoderma interaction have mainly focused on the response of the plant towards the fungus. In this study, a comparative proteomic analysis was conducted to investigate the change in protein expression of two Ganoderma spp., namely Ganoderma boninense, which is known as the most aggressive species and Ganoderma tornatum, previously reported as non-pathogenic. Our results showed that both species colonize and penetrate the oil palm root after only 72 h of inoculation. In addition, proteins were expressed differentially in both species that have either direct or indirect links to virulence and pathogenicity. Other proteins related to fungal growth, metabolism and stress from both species were also discussed in this study. Highlights: Both Ganoderma boninense and Ganoderma tornatum colonize oil palm roots after 72 h of inoculation. In both species, mycelial proteins from oil palm root exhibit different expression profiles when compared with the mycelia grown on PDA. The identified proteins were mainly involved in virulence and pathogenicity, metabolism, growth and maintenance of both Ganoderma species.