Effective UV/Ozone irradiation method for decontamination of hydroxyapatite surfaces. Issue 8 (August 2017)

• Record Type: Journal Article
• Authors: Yasuda, Keisuke; Okazaki, Yohei; Abe, Yasuhiko; Tsuga, Kazuhiro
• Is Part Of: Heliyon. Volume 3:Issue 8(2017)
• Publication Date: 2017-08
• Subjects: Engineering -- Bioengineering -- Materials science; Research -- Periodicals; Medical sciences -- Periodicals; Natural history -- Periodicals; Social sciences -- Periodicals; Earth sciences -- Periodicals; Physical sciences -- Periodicals; 507.2
• DOI: 10.1016/j.heliyon.2017.e00372
• Languages: English
• ISSNs: 2405-8440

Abstract

Abstract: The purpose of this study was to establish whether UV/ozone (O3 ) irradiation method can effectively decontaminate hydroxyapatite surfaces, including those modified by the treatment with 30% phosphoric acid solution through morphological and chemical surface analyses (surface roughness, X-ray photoelectron spectroscopy and wettability), and to evaluate the in vitro response of osteoblast-like MC3T3-E1 cells to the modified hydroxyapatite surface decontaminated via this method. The amount of carbon and the contact angle of hydroxyapatite surfaces were significantly decreased by UV/O3 irradiation that lasted for ≥ 5 and ≥ 3 min, respectively ( P < 0.01). Additionally, 7-day storage of H3 PO4 -modified hydroxyapatite surface decontaminated with 5-min irradiation did not affect contact angle values ( P > 0.05). MC3T3-E1 cell proliferation, differentiation (as assessed by relative ALP and OCN mRNA levels), and mineralisation were significantly promoted on irradiated surfaces ( P < 0.05). These findings show that UV/O3 irradiation for ≥ 5 min significantly decontaminated H3 PO4 -modified hydroxyapatite surface, improved its wettability, and facilitated osteoblast growth and function.