Improved biodegradation potential of chlorobenzene by a mixed fungal-bacterial consortium. (September 2017)

• Record Type: Journal Article
• Authors: Cheng, Zhuowei; Li, Chao; Kennes, Christian; Ye, Jiexu; Chen, Dongzhi; Zhang, Shihan; Chen, Jianmeng; Yu, Jianming
• Is Part Of: International biodeterioration & biodegradation. Volume 123(2017)
• Page Start: 276
• Page End: 285
• Publication Date: 2017-09
• Subjects: Mono-chlorobenzene -- Biodegradation -- Fungal-bacterial consortium -- Kinetic analysis -- Mineralization; Biodegradation -- Periodicals; Bioremediation -- Periodicals; Biodegradation -- Periodicals; Biodégradation -- Périodiques; Biorestauration -- Périodiques; Electronic journals; 620.11223
• DOI: 10.1016/j.ibiod.2017.07.008
• Languages: English
• ISSNs: 0964-8305

Abstract

Abstract: A defined consortium of Ralstonia pickettii L2 (bacterium) and Trichoderma viride LW-1 (fungus) was selected to assess its potential for the enhanced biodegradation of mono-chlorobenzene (CB). At an initial concentration of 220 mg L −1 CB, the developed consortium showed an enhanced degradation rate of 0.50 mg CB·g −1 protein ·h −1, while the individual Ralstonia sp. L2 and Trichoderma sp. LW-1 showed average degradation rates of 0.34 and 0.32 mg CB·g −1 protein ·h −1, respectively. A CO2 conversion level of up to 86.3% reflected a possible high mineralization extent of CB by the co-culture. The estimated μmax and vmax values were 0.36 h −1 and 0.41 h −1 for the consortium, which were much higher than the values obtained by each strain individually. 2-Chlorophenol (2-CP) accumulated in the growth medium of strain L2 and inhibited its growth, but it could be consumed quickly by the fungus LW-1, providing a possibility to reach complete biodegradation of CB in a short time. Real-time PCR revealed that bacterium L2 played a major role in the initial stage, and that fungus LW-1 grew well if 2-CP was generated. These results suggest that the fungal-bacterial consortium might be effectively applied for complete biodegradation of CB and have a potential environmental implication in purification of CB-contaminated environments. Graphical abstract: Highlights: First report on the use of a fungal-bacterial consortium for the biodegradation of CB. The biodegradation rate of consortium was 1.5 times more than that of single one. The addition of fungus LW-1 could relieve the inhibition of 2-CP to bacterium L2. RT-PCR analysis confirmed the strains growing with different rates at various times. The interactions within the different microorganisms led to the enhanced biodegradation.