Pseudomonas aeruginosa auto inducer3-oxo-C12-HSL exerts bacteriostatic effect and inhibits Staphylococcus epidermidis biofilm. (September 2017)

• Record Type: Journal Article
• Authors: Singh, Pradeep Kumar; Yadav, Vivek Kumar; Kalia, Manmohit; Dohare, Suhaga; Sharma, Deepmala; Agarwal, Vishnu
• Is Part Of: Microbial pathogenesis. Volume 110(2017)
• Page Start: 612
• Page End: 619
• Publication Date: 2017-09
• Subjects: Co-infection -- P. aeruginosa -- S. epidermidis -- AHLs -- Quorum Sensing -- Biofilms; AHL acyl homoserine lactone -- EPS extracellular polymorphic substances -- C4-HSL N-Butyryl-l-homoserine lactone -- 3-oxo-C12–HSL N-(3-Oxododecanoyl)-l-homoserine lactone -- FITC-Con A Fluorescein isothiocyanate-concanavalin A -- PI Propidium iodide -- DAPI 4′, 6-diamidino-2-phenylindole; Pathogenic microorganisms -- Periodicals; Pathology, Molecular -- Periodicals; Communicable Diseases -- microbiology -- Periodicals; Communicable Diseases -- parasitology -- Periodicals; Micro-organismes pathogènes -- Périodiques; Pathologie moléculaire -- Périodiques; Electronic journals; 616.9041
• DOI: 10.1016/j.micpath.2017.08.009
• Languages: English
• ISSNs: 0882-4010

Abstract

Abstract: Pseudomonas aeruginosa has evolved the 3-oxo-C12 -HSL and C4 -HSL based quorum sensing system which is responsible for the regulation of various virulence factors and helps to dominates over other bacterial species. Staphylococcus epidermidis has frequently been reported with P. aeruginosa while the role of C4 -HSL and 3-oxo-C12 –HSL on the S. epidermidis had widely been unexplored, and as per our knowledge, this is the first report on the impact of C4 -HSL and 3-oxo-C12 –HSL over S. epidermidis growth and biofilm. We found that among the two AHL molecules; only 3-oxo-C12 –HSL was able to exert a significant effect in all the experiments including growth and biofilm of S. epidermidis . 3-oxo-C12 –HSL at 100 μM and 200 μM concentrations were able to initiate the apparent transient type of planktonic growth inhibition in S. epidermidis . Microscopic analysis and biofilm quantification assay showed the inhibitory effect of 3-oxo-C12 –HSL against S. epidermidis biofilm, initial attachment, and EPS production. The study concludes that P. aeruginosa associated 3-oxo-C12 -HSL exerts the inhibitory effect on S. epidermidis growth and biofilms and thus it may also help Pseudomonas to dominate under the co-infection conditions. Graphical abstract: Highlights: Out of two P. aeruginosa associated major quorum molecules 3-oxo-C12 -HSL affects S. epidermidis growth and biofilm while C4 -HSL do not affect the same. 3-oxo-C12 -HSL causes transient pattern of growth inhibition in S. epidermidis . 3-oxo-C12 -HSL showed inhibitory impact on S. epidermidis initial adhesion and EPS production.