Molecular detection of Isoniazid, Rifampin and Ethambutol resistance to M. tuberculosis and M. bovis in multidrug resistant tuberculosis (MDR-TB) patients in Pakistan. (September 2017)

• Record Type: Journal Article
• Authors: Munir, Saba; Mahmood, Nasir; Shahid, Saman; Khan, Muhammad Idrees
• Is Part Of: Microbial pathogenesis. Volume 110(2017)
• Page Start: 262
• Page End: 274
• Publication Date: 2017-09
• Subjects: Drug proportion (DP) method -- Mycobacterial growth indicator tube (MGIT) -- Multiple allele specific (MAS) -PCR -- hupB -- katG -- rpoB -- embB -- Isoniazid (INH) -- Rifampin (RIF) -- Ethambutol (EMB); Pathogenic microorganisms -- Periodicals; Pathology, Molecular -- Periodicals; Communicable Diseases -- microbiology -- Periodicals; Communicable Diseases -- parasitology -- Periodicals; Micro-organismes pathogènes -- Périodiques; Pathologie moléculaire -- Périodiques; Electronic journals; 616.9041
• DOI: 10.1016/j.micpath.2017.07.005
• Languages: English
• ISSNs: 0882-4010

Abstract

Abstract: The various aspects of MDR-TB, type of pathogen, different drug sensitive methods and mutation (s) in specific genes were determined. The histone-like protein ( hupB ) gene of M. tuberculosis was targeted by using primer sets: N & S and M & S (produced 645 bp & 318 bp fragment respectively). The most significant risk factors were the poverty and male gender of ages 11–25 years. All samples were detected as M. tuberculosis . By Drug Proportion method, the highest percentage (37%) was found resistant to only Rifampin. By MGIT method, the highest percentage (82.2%) was found resistant with the triple combination (Rifampin-RIF + Isoniazid-INH + Ethambutol-EMB) of the drugs. The highest mutations (76.92%) were found in gene rpoB (codon 531) in MDR TB patients. By, MAS-PCR, the highest percentage (34%) were found resistant to combination (INH + RIF) of the drugs. Minimum samples were resistant to RIF and RIF + INH drugs by MGIT, while proportionate results were observed from MAS-PCR and DP. Moreover, by MAS-PCR mutation in gene embB (306) caused EMB resistance (51.64%). We found that M. tuberculosis was the main cause of MDR-TB. Our findings may further be used for an early diagnosis of multi-drug resistant tuberculosis. Highlights: The histone-like protein ( hupB ) gene of M. tuberculosis was targeted by using two primer sets. By Drug Proportion method, the highest percentage (37%) was found resistant to only RIF. By MGIT method, the highest percentage (82.2%) was resistant with the triple combination of drugs. The highest mutations (76.92%) were found with gene rpoB (531). By MAS-PCR mutation in gene embB (306) caused EMB resistance (51.64%).