In vitro expression of Sec-dependent pathway and type 4B secretion system in Piscirickettsia salmonis. (September 2017)

• Record Type: Journal Article
• Authors: Cortés, Marcos; Sánchez, Patricio; Ruiz, Pamela; Haro, Ronie; Sáez, Jerson; Sánchez, Fabián; Hernández, Mauricio; Oliver, Cristian; Yáñez, Alejandro J.
• Is Part Of: Microbial pathogenesis. Volume 110(2017)
• Page Start: 586
• Page End: 593
• Publication Date: 2017-09
• Subjects: Piscirickettsia salmonis -- SRS -- Sec-dependent pathway -- Type 4 secretion system -- T4BSS -- dot/icm; Pathogenic microorganisms -- Periodicals; Pathology, Molecular -- Periodicals; Communicable Diseases -- microbiology -- Periodicals; Communicable Diseases -- parasitology -- Periodicals; Micro-organismes pathogènes -- Périodiques; Pathologie moléculaire -- Périodiques; Electronic journals; 616.9041
• DOI: 10.1016/j.micpath.2017.08.003
• Languages: English
• ISSNs: 0882-4010

Abstract

Abstract: Piscirickettsia salmonis is an intracellular bacterium and the causative agent of Piscirickettsiosis, a disease responsible for considerable mortalities in the Chilean salmon farming industry. Currently, P. salmonis protein translocation across the membrane and the mechanisms by which virulence factors are delivered to host cells are poorly understood. However, it is known that Gram-negative bacteria possess several mechanisms that transport proteins to the periplasmic and extracellular compartments. The aim of this study was to evaluate the expressional changes of several genes in the P. salmonis Sec-dependent pathway and type 4B secretion system during in vitro infection. Genes homologous and the main proteins belonging to Sec-dependent pathway and Type 4 Dot/Icm secretion system were found in the genome and proteome of P. salmonis AUSTRAL-005 strain. Additionally, several genes of these protein transport mechanisms were overexpressed during in vitro P. salmonis infection in SHK-1 cell line. The obtained data indicate that the Sec-dependent pathway and Type 4B secretion system are biologically active during P. salmonis infection. These mechanisms could contribute to the recycling of proteins into the inner and outer bacterial membrane and in translocate virulence factors to infected cell, which would favor the structural integrity and virulence of this bacterium. Highlights: P. salmonis contained genes of Sec-dependent pathway and T4BSS. yajC, yidC and dotG/dotH transcript levels were overexpressed during an infection. The holotranslocon SecDF/YajC and T4BSS core proteins were identified in P. salmonis.