One‐day pulsed‐field gel electrophoresis protocol for rapid determination of emetic Bacillus cereus isolates. Issue 7 (20th March 2015)

• Record Type: Journal Article
• Authors: Kaminska, Paulina S.; Fiedoruk, Krzysztof; Jankowska, Dominika; Mahillon, Jacques; Nowosad, Karol; Drewicka, Ewa; Zambrzycka, Monika; Swiecicka, Izabela
• Is Part Of: Electrophoresis. Volume 36:Issue 7/8(2015)
• Page Start: 1051
• Page End: 1054
• Publication Date: 2015-03-20
• Subjects: Bacillus cereus -- Emetic isolates -- PFGE -- Rapid protocol; Electrophoresis -- Periodicals; Electrophoresis -- Periodicals; 541.372
• DOI: 10.1002/elps.201400552
• Languages: English
• ISSNs: 0173-0835

Abstract

Abstract : Bacillus cereus, the Gram‐positive and spore‐forming ubiquitous bacterium, may cause emesis as the result of food intoxication with cereulide, a heat‐stable emetic toxin. Rapid determination of cereulide‐positive B. cereus isolates is of highest importance due to consequences of this intoxication for human health and life. Here we present a 1‐day pulsed‐field gel electrophoresis for emetic B. cereus isolates, which allows rapid and efficient determination of their genomic relatedness and helps determining the source of intoxication in case of outbreaks caused by these bacilli.