Polysaccharides of Dendrobium officinale Kimura & Migo protect gastric mucosal cell against oxidative damage-induced apoptosis in vitro and in vivo. (17th August 2017)

• Record Type: Journal Article
• Authors: Zeng, Qiang; Ko, Chun-Hay; Siu, Wing-Sum; Li, Long-Fei; Han, Xiao-Qiang; Yang, Liu; Bik-San Lau, Clara; Hu, Jiang-Miao; Leung, Ping-Chung
• Is Part Of: Journal of ethnopharmacology. Volume 208(2017)
• Page Start: 214
• Page End: 224
• Publication Date: 2017-08-17
• Subjects: DO Dendrobium officinale Kimura & Migo -- DOP Dendrobium officinale Kimura & Migo polysaccharides -- H2O2 Hydrogen peroxide -- NSAIDs Non-steroidal anti-inflammatory drugs -- GSH Glutathione -- COX2 Cyclooxygenase-2 -- ROS Reactive oxygen species -- iNOS Inducible NO synthase -- MCP-1 Monocyte chemoattractant protein-1 -- HPGPC High performance gel permeation chromatography -- PAS Periodic acid-Schiff -- PARP Poly ADP-ribose polymerase; Polysaccharides -- Dendrobium officinale Kimura & Migo -- Gastric injury -- Oxidative damage -- Apoptosis; Ethnopharmacology -- Periodicals; Pharmacognosy -- Periodicals; Herbs -- Periodicals; Herbs -- Periodicals; Pharmacognosy -- Periodicals; Pharmacognosie -- Périodiques; Herbes -- Périodiques; 615.1
• DOI: 10.1016/j.jep.2017.07.006
• Languages: English
• ISSNs: 0378-8741

Abstract

Abstract: Ethnopharmacological relevance: Dendrobium officinale Kimura & Migo (DO) is a valuable Traditional Chinese Medicine to nourish stomach, in which polysaccharides are identified as active ingredients. However, limited scientific evidences have been reported on the gastroprotective efficacy of DO. The aim of the current study was to investigate the protective effects and underlying mechanism of polysaccharides from DO(DOP) on gastric mucosal injury. Material and methods: For in vitro study, HFE145 cells were pretreated with DOP before induction of cell apoptosis by H2 O2 . Cell apoptosis and related proteins expression were detected. In the in vivo study, absolute ethanol was administered orally to induce gastric mucosal injury in rat. The gastric mucosal injury area and histological examination were used to evaluate the effects of DOP treatment on the recovery of the gastric mucosal injury. Results: H2 O2 treatment for 6 h significantly induced cell apoptosis in HFE145 cells. However, the destructive effects of H2 O2 on HFE 145 cells could be reversed by the pretreatment with DOP. The increased ROS level induced by H2 O2 for 4 h was reduced after DOP pretreatment. The number of apoptotic cells in both early and late apoptosis stages decreased significantly and the nuclei morphology changes were improved with DOP pretreatment. Furthermore, DOP inhibited caspase 3 activation and PARP cleavage, downregulated Bax expression and upregulated Bcl2 expression in cell model. Further study revealed that pretreatment of DOP inhibited p -NF-κBp65/NF-κBp65 level, indicating DOP inhibited H2 O2 -mediated apoptosis via suppression of NF-κB activation. In addition, DOP treatment could ameliorate gastric mucosal injury and inhibit mucin loss induced by ethanol in animal model. DOP treatment also interfered with ethanol-induced apoptosis process by downregulating Bax/Bcl2 ratio in gastric mucosa. Conclusions: The present study was the first one to demonstrate the gastroprotective effect of DOP through inhibiting oxidative stress-induced apoptosis. This study provided a solid evidence for the potential use of DO as a therapy or health supplement for gastric mucosal diseases Graphical abstract: