(p-ClPhSe)2 stimulates carbohydrate metabolism and reverses the metabolic alterations induced by high fructose load in rats. (September 2017)

• Record Type: Journal Article
• Authors: Quines, Caroline B.; Rosa, Suzan G.; Chagas, Pietro M.; Velasquez, Daniela; Prado, Vinicius C.; Nogueira, Cristina W.
• Is Part Of: Food and chemical toxicology. Volume 107:Part A(2017)
• Page Start: 122
• Page End: 128
• Publication Date: 2017-09
• Subjects: Organoselenium -- Diselenide -- Fructose -- Insulin-mimetic action -- Metabolism; Toxicology -- Periodicals; Food poisoning -- Periodicals; Food Poisoning -- Periodicals; Toxicology -- Periodicals; Toxicologie -- Périodiques; Intoxications alimentaires -- Périodiques; Food poisoning; Toxicology; Periodicals; Electronic journals; 615.9
• DOI: 10.1016/j.fct.2017.06.038
• Languages: English
• ISSNs: 0278-6915

Abstract

Abstract: The modern life leads to excess consumption of food rich in fructose; however, the long-term changes in carbohydrate and lipid metabolism could lead to metabolic dysfunction in humans. The present study evaluated the in vitro insulin-mimetic action of p -chloro-diphenyl diselenide ( p -ClPhSe)2 . The second aim of this study was to investigate if ( p -ClPhSe)2 reverses metabolic dysfunction induced by fructose load in Wistar rats. The insulin-mimetic action of ( p -ClPhSe)2 at concentrations of 50 and 100 μM was determined in slices of rat skeletal muscle. ( p -ClPhSe)2 at a concentration of 50 μM stimulated the glucose uptake by 40% in skeletal muscle. A dose-response curve revealed that ( p -ClPhSe)2 at a dose of 25 mg/kg reduced (∼20%) glycemia in rats treated with fructose (5 g/kg, i.g.). The administration of fructose impaired the liver homeostasis and ( p -ClPhSe)2 (25 mg/kg) protected against the increase (∼25%) in the G-6-Pase and isocitrate dehydrogenase activities and reduced the triglyceride content (∼25%) in the liver. ( p -ClPhSe)2 regulated the liver homeostasis by stimulating hexokinase activity (∼27%), regulating the TCA cycle activity (increased the ATP and citrate synthase activity (∼15%)) and increasing the glycogen levels (∼67%). In conclusion, ( p -ClPhSe)2 stimulated carbohydrate metabolism and reversed metabolic dysfunction in rats fed with fructose. Highlights: ( p -ClPhSe)2 had an insulin-mimetic action in vitro. ( p -ClPhSe)2 reduced glycemia increase in fructose-fed rats. ( p -ClPhSe)2 stimulates carbohydrate metabolism in fructose-fed rats. ( p -ClPhSe)2 restored the hepatic metabolic homeostasis in fructose-fed rats.