Liposome-supported enzymatic peritoneal dialysis. (November 2017)

• Record Type: Journal Article
• Authors: Pratsinis, Anna; Zuercher, Stefanie; Forster, Vincent; Fischer, Eric J.; Luciani, Paola; Leroux, Jean-Christophe
• Is Part Of: Biomaterials. Volume 145(2017)
• Page Start: 128
• Page End: 137
• Publication Date: 2017-11
• Subjects: Bionanotechnology -- Peritoneal dialysis -- Alcohol intoxication -- Enzyme delivery -- Proteoliposomes; Biomedical materials -- Periodicals; Biocompatible Materials -- Periodicals; Biomatériaux -- Périodiques; 610.28
• DOI: 10.1016/j.biomaterials.2017.08.016
• Languages: English
• ISSNs: 0142-9612

Abstract

Abstract: Compared to hemodialysis, peritoneal dialysis represents a more straightforward and less invasive alternative, though current solutions are not as effective. Herein, the feasibility of liposome-supported enzymatic peritoneal dialysis (LSEPD) is explored to increase the functionality of peritoneal dialysis for the model indication acute alcohol poisoning. Enzyme-loaded liposomes (E-Liposomes) containing alcohol metabolizing enzymes, alcohol oxidase and catalase, are developed and their in vitro and in vivo performances investigated. The E-Liposomes outperform the free enzymes in stability, overcoming the thermal instability of alcohol oxidase and enhancing the in vitro ethanol elimination, which is further accelerated by hydrogen peroxide, due to the rapid generation of oxygen by catalase. Compared to the free enzymes, the E-Liposomes exhibit reduced systemic exposure and organ distribution. In a rodent ethanol intoxication model, LSEPD enhances ethanol metabolism as evidenced by an increased acetaldehyde production, ethanol's primary metabolite. In conclusion, LSEPD presents an innovative platform to temporarily enhance xenobiotic metabolism, in view of the improved enzyme stability and peritoneal retention. Graphical abstract: Liposome-supported enzymatic peritoneal dialysis is developed for the model indication acute alcohol poisoning. Enhanced enzymatic stability and low systemic exposure of the administered enzymes is revealed, overall contributing to improved ethanol metabolism. Current findings demonstrate the ability to carry out chemical reactions in the peritoneal space and propose a novel modality to temporarily enhance xenobiotic metabolism.