Anti-proliferative activity of Gum kondagogu (Cochlospermum gossypium)-gold nanoparticle constructs on B16F10 melanoma cells: An in vitro model. (July 2017)

• Record Type: Journal Article
• Authors: Kalaignana Selvi, S.; Mahesh Kumar, Jerald; Sashidhar, R.B.
• Is Part Of: Bioactive carbohydrates and dietary fibre. Volume 11(2017)
• Page Start: 38
• Page End: 47
• Publication Date: 2017-07
• Subjects: Gum kondagogu -- GK-Au nanoparticle construct -- Anti-proliferative potential -- Gene expression; Functional foods -- Periodicals; Fiber in human nutrition -- Periodicals; Carbohydrates -- Periodicals; Glycoproteins -- Periodicals; Functional Food -- Periodicals; Dietary Fiber -- Periodicals; Carbohydrates -- Periodicals; Carbohydrates; Fiber in human nutrition; Functional foods; Glycoproteins; Periodicals; 613.28
• DOI: 10.1016/j.bcdf.2017.07.002
• Languages: English
• ISSNs: 2212-6198

Abstract

Abstract: The present study has explored the reducing and capping potentials of gum kondagogu (GK) for the synthesis of gold nanoparticle constructs. The formation of gold nanoparticles was confirmed by UV- absorption, FTIR, AFM, SEM and TEM. The GK-AuNPs showed a characteristic absorption peak at 534 nm in UV spectra with the size range of 4.08–12.73 ± 0.75 nm. As a result of wide screening on the application of newly synthesized GK-AuNPs their anti-proliferative potential were discovered by MTT assay, confocal microscopy, flow cytometry and quantitative real time PCR techniques. MTT assay of synthesized GK-AuNPs exhibited IC50 concentration of 1.7 µg L −1 after 48 h. FACS analysis revealed that cell cycle gets arrested at G1 to S phase, with up-regulation of p53, caspase-3, caspase-9, PPARa and PPARb genes and down regulation of Bcl-2 and Bcl-x(K) genes, in B16F10 cells treated with GK-AuNPs confirming the anti-proliferative properties of the nanoparticles. Graphical abstract: Highlights: Gum kondagogu, a non-toxic biopolymer material mediated synthesis of GK-AuNPs. GK-AuNPs activity was assessed by MTT, Flow cytometer, and confocal microscopy. Mechanism of action is by activation of p53, caspase-9 and, ultimately caspase-3.