Surface modification of PS microtiter plate with chitosan oligosaccharides by 60Co irradiation. (15th November 2017)

• Record Type: Journal Article
• Authors: Han, Shixin; Wang, Huashan; Sun, Zhipeng; Zhao, Huiqi; Zhang, Pai
• Is Part Of: Carbohydrate polymers. Volume 176(2017)
• Page Start: 135
• Page End: 139
• Publication Date: 2017-11-15
• Subjects: Microtiter plate -- Irradiation grafting -- Chitosan-oligosaccharides -- Protein adsorption; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2017.08.066
• Languages: English
• ISSNs: 0144-8617

Abstract

Highlights: A γ-ray irradiation grafting method of chitosan oligosaccharide (COS) is proposed. The chitosan oligosaccharide concentration of immersion solution has obvious influence on the properties of the polystyrene microtiter's inner surface. The PS-COS microtiter plates can adsorb the BSA homogeneously. The absorbance variances of the COS modified plate is less than 5% while the BSA adsorption is higher than 230 ng cm −2 . Abstract: By 60 Co-γ irradiation method, the Chitosan-oligosaccharide (COS) was grafted onto the inner surface of the polystyrene (PS) microtiter, which was soaked with COS solution before the irradiation. To evaluated the effect of COS concentration on the properties of the PS microtiter, FTIR, XPS, AFM, Contact angle tester and enzyme-linked analyser was used to measure the surface properties and BSA adsorption of PS-COS plates. The results shows that, with the increase of COS concentration, the contact angle clearly decreased at the dose of 12 kGy. The absorbance variances of the COS modified plate is less than 5% while the BSA adsorption is higher than the PS plates. The COS-modified microtiter has the potential applications in biochemical analysis.