Addition of Pullulan to Trehalose Glasses Improves the Stability of β-Galactosidase at High Moisture Conditions. (15th November 2017)

• Record Type: Journal Article
• Authors: Teekamp, Naomi; Tian, Yu; Visser, J. Carolina; Olinga, Peter; Frijlink, Henderik W.; Woerdenbag, Herman J.; Hinrichs, Wouter L.J.
• Is Part Of: Carbohydrate polymers. Volume 176(2017)
• Page Start: 374
• Page End: 380
• Publication Date: 2017-11-15
• Subjects: BSA bovine serum albumin -- DSC differential scanning calorimetry -- DVS dynamic vapor sorption -- HEPES 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid -- RH relative humidity -- Tg glass transition temperature -- Tg′ glass transition temperature of the maximally freeze concentrated fraction; pullulan -- trehalose -- protein stabilization -- sugar glass -- dynamic vapor sorption -- differential scanning calorimetry; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2017.08.084
• Languages: English
• ISSNs: 0144-8617

Abstract

Highlights: Amorphous freeze-dried pullulan displays an exceptionally high Tg of 261 °C. Even at 90% humidity, the Tg of freeze-dried pullulan remains above room temperature. Pullulan/trehalose blends are homogeneous and Tg (′)s obey the Gordon-Taylor equation. Pullulan (>50% content) prevents crystallization of trehalose at high RH conditions. Pullulan/trehalose blends provide better protein stability at high RH than trehalose Abstract: Incorporation of therapeutic proteins in a matrix of sugar glass is known to enhance protein stability, yet protection is often lost when exposed to high relative humidity (RH). We hypothesized that especially in these conditions the use of binary glasses of a polysaccharide and disaccharide might yield advantages for protein stability. Therefore, different amounts of the polysaccharide pullulan were introduced in freeze-dried trehalose glasses. In these homogeneous blends, the presence of pullulan above 50 weight % prevented crystallization of trehalose when exposed to high RH. Storage stability testing up to 4 weeks of the model protein β-galactosidase incorporated in pullulan/trehalose blends showed superior behavior of pure trehalose at 30 °C/0% RH, while pullulan/trehalose blends yielded the best stability at 30 °C/56% RH. In conclusion, binary glasses of pullulan and trehalose may provide excellent stability of proteins under storage conditions that may occur in practice, namely high temperature and high RH.