Characterization of xylan in the early stages of secondary cell wall formation in tobacco bright yellow-2 cells. (15th November 2017)

• Record Type: Journal Article
• Authors: Ishii, Tadashi; Matsuoka, Keita; Ono, Hiroshi; Ohnishi-Kameyama, Mayumi; Yaoi, Katsuro; Nakano, Yoshimi; Ohtani, Misato; Demura, Taku; Iwai, Hiroaki; Satoh, Shinobu
• Is Part Of: Carbohydrate polymers. Volume 176(2017)
• Page Start: 381
• Page End: 391
• Publication Date: 2017-11-15
• Subjects: 2-AB 2-aminobenzamide -- BY-2 Bright Yellow-2 -- DEX dexamethasone -- EG endo-(1 → 4)-glucanase -- EPG endo-polygalacturonase -- ESI-MS electrospray ionization mass spectrometry -- LC liquid chromatography -- LC-ESI-MS liquid chromatograph-electrospray ionization mass spectrometry -- MALDI-TOF MS matrix assisted laser desorption/ionization time of flight mass spectrometry -- NAC NAM/ATAF/CUC -- RG-I rhamnogalacturonan I -- RG-II rhamnogalacturonan II -- TMS trimethylsilyl -- XG xyloglucan -- VND7 vascular-related NAC domain7; Cell wall polysaccharides -- Glucuronic acid -- Inducible VND7 system -- Tobacco BY-2 cell -- Xylan; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2017.08.108
• Languages: English
• ISSNs: 0144-8617

Abstract

Highlights: Cell walls polysaccharides differentiating to xylem vessel elements were characterized. A reducing end sequence of xylan is present in primary and secondary cell walls. Xylan changes in structure and amounts during the early stages of secondary wall formation. Abstract: The major polysaccharides present in the primary and secondary walls surrounding plant cells have been well characterized. However, our knowledge of the early stages of secondary wall formation is limited. To address this, cell walls were isolated from differentiating xylem vessel elements of tobacco bright yellow-2 (BY-2) cells induced by VASCULAR-RELATED NAC-DOMAIN7 (VND7). The walls of induced VND7-VP16-GR BY-2 cells consisted of cellulose, pectic polysaccharides, hemicelluloses, and lignin, and contained more xylan and cellulose compared with non-transformed BY-2 and uninduced VND7-VP16-GR BY-2 cells. A reducing end sequence of xylan containing rhamnose and galaturonic acid- residues is present in the walls of induced, uninduced, and non-transformed BY-2 cells. Glucuronic acid residues in xylan from walls of induced cells are O -methylated, while those of xylan in non-transformed BY-2 and uninduced cells are not. Our results show that xylan changes in chemical structure and amounts during the early stages of xylem differentiation.