Synthesis of synthetic mannan backbone polysaccharides found on the surface of Mycobacterium tuberculosis as a vaccine adjuvant and their immunological properties. (1st November 2017)

• Record Type: Journal Article
• Authors: Wattanasiri, Chakree; Paha, Jiraporn; Ponpuak, Marisa; Ruchirawat, Somsak; Boonyarattanakalin, Siwarutt
• Is Part Of: Carbohydrate polymers. Volume 175(2017)
• Page Start: 746
• Page End: 755
• Publication Date: 2017-11-01
• Subjects: TB tuberculosis -- Mtb Mycobacterium tuberculosis -- LM lipomannan -- LAM lipoarabinomannan -- LPS lipopolysaccharide -- GPC gel permeable chromatography; ​mannan back-bone polysaccharides -- mannosyl tricyclic orthoester monomer -- Mycobacterium tuberculosis surface -- lipomannan -- vaccine adjuvant; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2017.07.045
• Languages: English
• ISSNs: 0144-8617

Abstract

Graphical abstract: Highlights: The Mtb mannan is made available in a rapid fashion for the first time. The rapid synthesis is done by a size, stereo, and regio-controlled polymerization. The size of the LM polysaccharide can be controlled by the monomer concentration. Strong in vitro adjuvant activities of the synthetic LM polysaccharide are revealed. Abstract: Surface components of Mycobacterium tuberculosis (Mtb) play crucial roles in modulating host immune responses. Thorough understandings of immunological properties of the Mtb's surface components are essential for the development of tuberculosis treatment and prevention. Unfortunately, the accessibility to the molecules on the surface of Mtb is limited by the structural complexity due to their various macromolecular nature and the hazard of culturing Mtb. In this study, we reveal a practical synthesis of lipomannan (LM) backbone polysaccharides – the core glycans found on Mtb's surface. A rapid synthetic approach based on a controlled polymerization was developed for the chemical synthesis of mannopyranans, the core structure of LM. The size of the LM glycans can be controlled by using specific monomer concentrations in addition to stereo- and regioselectivity derived from the versatile tricyclic orthoester mannose monomer. The immunological properties of the synthesized mannopyranans were investigated and their adjuvant potential was revealed. The adjuvanticity mechanism of the synthetic mannopyranans appears to involve the NF-κB and inflammasome pathways.