The inhibition of LPS-induced inflammation in RAW264.7 macrophages via the PI3K/Akt pathway by highly N-acetylated chitooligosaccharide. (15th October 2017)

• Record Type: Journal Article
• Authors: Xu, Qingsong; Liu, Meisi; Liu, Qishun; Wang, Wenxia; Du, Yuguang; Yin, Heng
• Is Part Of: Carbohydrate polymers. Volume 174(2017)
• Page Start: 1138
• Page End: 1143
• Publication Date: 2017-10-15
• Subjects: COS chitooligosaccharide -- NACOS N, acetylated chitooligosaccharide -- LPS lipopolysaccharides -- IL-6 interleukin-6 -- TNF-α tumor necrosis factor-α -- ROS reactive oxygen species -- NO nitric oxide -- PI3K phosphatidylinositol 3-kinase -- MW molecular weight -- COX-2 cyclooxygenase-2 -- ACL anterior cruciate ligament -- NF-κB nuclear factor-κB -- AP-1 activator protein-1 -- DMAP 4-dimethylaminopyridine -- FTIR Fourier transform infrared spectroscopy -- ESI–MS electrospray ionization mass spectrometry -- HPLC high performance liquid chromatography -- DP degree of polymerization -- SCOSs sulfated chitooligosaccharide -- AE-COS aminoethyl chitooligosaccharide; N-acetylated chitooligosaccharide -- Lipopolysaccharides -- Interleukin-6 -- Tumor necrosis factor-α -- Inflammation -- PI3K/Akt pathway; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2017.07.051
• Languages: English
• ISSNs: 0144-8617

Abstract

Highlights: NACOS suppressed LPS-induced inflammatory response in macrophages. NACOS repressed LPS-initiated phosphorylation of PI3K and Akt. NACOS could be as a promising candidate for novel inhibitor of inflammation. Abstract: Chitooligosaccharide (COS) has been shown to regulate many biological functions, such as antimicrobial effect and antitumor activity. In the present study, highly N-acetylated chitooligosaccharide (NACOS) was prepared by N-acetylation of COS, and the anti-inflammatory activity of NACOS in macrophages were evaluated. The results indicated NACOS significantly suppressed the LPS-induced pro-inflammatory cytokines interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) expression. Furthermore, the increased levels of reactive oxygen species (ROS) and nitric oxide (NO) were repressed by NACOS in a dose dependent manner. However, NACOS itself had no significant effect on the cell viability and cellular morphology. Signal transduction studies demonstrated that NACOS remarkably inhibited LPS-enhanced phosphorylation of phosphatidylinositol 3-kinase (PI3K) and Akt. These findings provide a possible molecular mechanism by which NACOS inhibit LPS-induced inflammatory response in macrophages, and a basis for utilizing NACOS in pharmaceutical therapy against inflammation.