Characterization and anticoagulant activity of a fucosylated chondroitin sulfate with unusually procoagulant effect from sea cucumber. (15th October 2017)

• Record Type: Journal Article
• Authors: Ben Mansour, Mohamed; Balti, Rafik; Ollivier, Véronique; Ben Jannet, Hichem; Chaubet, Frédéric; Maaroufi, Raoui Mounir
• Is Part Of: Carbohydrate polymers. Volume 174(2017)
• Page Start: 760
• Page End: 771
• Publication Date: 2017-10-15
• Subjects: Fucosylated chondroitin sulfate -- Sea cucumber -- Holothuria polii -- Anticoagulant activity -- Thrombin generation; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2017.06.128
• Languages: English
• ISSNs: 0144-8617

Abstract

Highlights: The fucosylated chondroitin sulfate was purified from sea cucumber Holothuria polii . The physicochemical and structural characteristics of FuCS were investigated. FuCS has high sulfate content and a relatively low average molecular mass. The FuCS exhibited a high anticoagulant effect mediated by both HCII and AT. FuCS represent a naturally alternative for first generation anticoagulants. Abstract: The fucosylated chondroitin sulfate (FuCS) was isolated from the sea cucumber Holothuria polii body wall and then purified by anion exchange chromatography and the structure was characterized by FT-IR and NMR spectroscopy. Anticoagulant activity was measured in plasma by classical anticoagulation tests and the thrombin generation was assessed by calibrated automated thrombography. The results showed that the FuCS obtained at a yield of 4.66% possesses high sulfate content 43% and an average molecular mass of 45.8 kDa. Structural analysis revealed the presence of alternating units of chondroitine sulfate branched at position 3 of the β-d -glucuronic acid by two types of fucose 2, 4- O -di and 3, 4- O -disulfated residues. The FuCS exhibited a high anticoagulant effect mediated essentially by heparin cofactor II and to lesser extent by antithrombin. Moreover, the CAT assay depicted a dual effect of FuCS with a procoagulant tendency for low doses and an anticoagulant effect at higher doses.