A novel self-assembly Lentinan-tetraphenylethylene​ composite with strong blue​ fluorescence in water and its properties. (15th October 2017)

• Record Type: Journal Article
• Authors: Li, Mengxia; Chen, Pan; Xu, Min; Xu, Xiaojuan
• Is Part Of: Carbohydrate polymers. Volume 174(2017)
• Page Start: 13
• Page End: 24
• Publication Date: 2017-10-15
• Subjects: Tetraphenylethylene (PubChem CID: 69437) -- Dimethyl sulfoxide (PubChem CID: 679) -- 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyl tetrazolium bromide (PubChem CID: 64965) -- Dimethyl sulfoxide-d6 (PubChem CID: 75151); t-LNT triple helical lentinan -- TPE tetraphenylethylene -- AIE aggregation induced emission -- FDA food and drug administration from U.S.A -- DMSO dimethyl sulfoxide -- FONs fluorescent organic nanoparticles -- s-LNT single chain of lentinan -- r-LNT renatured triple helical lentinan -- AFM atom force microscopy -- TEM transmission electron microscopy -- MD molecular dynamic -- MTT 3-[4, 5-dimethylthiazol-2-yl]-2 5-diphenyl tetrazolium bromide -- RCF relative centrifugal force -- DMEM Dulbecco's modified eagle's medium -- PBS phosphate-buffered saline -- CCTCC China center for typical culture collection -- IR infrared spectroscopy -- NMR nuclear magnetic resonance -- PBC periodic boundary condition -- CAC critical aggregation concentration -- FBS fatal bovine serum -- SCG scleroglucan -- SPG schizophyllan -- CUR curdlan -- RIR restriction of intramolecular rotations -- DNTs dendritic nanotubes; Triple helical β-(1→3)-glucan -- Self-assembly -- Tetraphenylethylene -- Blue fluorescence -- Lentinus edodes; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2017.06.013
• Languages: English
• ISSNs: 0144-8617

Abstract

Graphical abstract: Highlights: t-LNT self-assembled into dendrite-like aggregates via parallel alignment in water. t-LNT enhanced the blue fluorescence of TPE in water. t-LNT/TPE showed high water-dispersibility, good stability and biocompatibility. Abstract: We report a unique self-assembly of lentinan, a triple helical β-(1→3)-glucan (t-LNT), in water. By molecular dynamics simulation, it was found that t-LNT aggregated preferentially along the chain direction to form long chains, accompanied by side-direction linkage to form branches. Transmission electron microscopy images demonstrated that t-LNT formed dendrite-like fibers, which further formed fishnet-like porous/mesoporous aggregates with increasing concentration. The meshes in the fishnet were ascribed to the intersection of branches. The major driving force for aggregation was expected to be hydrogen bonding between hydroxyl groups in t-LNT chains. Based on this self-assembly behavior, a novel composite was prepared from t-LNT and tetraphenylethylene (TPE) by entrapping TPE aggregates into the meshes of t-LNT fishnets. The as-prepared t-LNT/TPE composite largely enhanced the blue fluorescence of TPE in water, exhibiting stable optical property and good biocompatibility, and t-LNT is expected to show great potential as a carrier of hydrophobic molecules for biomedical application.