Zinc oxide nanoparticle toxicity in embryonic zebrafish: Mitigation with different natural organic matter. (November 2017)

• Record Type: Journal Article
• Authors: Kteeba, Shimaa M.; El-Adawi, Hala I.; El-Rayis, Osman A.; El-Ghobashy, Ahmed E.; Schuld, Jessica L.; Svoboda, Kurt R.; Guo, Laodong
• Is Part Of: Environmental pollution. Volume 230(2017)
• Page Start: 1125
• Page End: 1140
• Publication Date: 2017-11
• Subjects: Nanotoxicity -- Dissolved organic matter -- ZnO-NPs -- Embryonic zebrafish -- Mitigation -- Natural organic matter; Pollution -- Periodicals; Pollution -- Environmental aspects -- Periodicals; Environmental Pollution -- Periodicals; Pollution -- Périodiques; Pollution -- Aspect de l'environnement -- Périodiques; Pollution -- Effets physiologiques -- Périodiques; Pollution; Pollution -- Environmental aspects; Periodicals; Electronic journals; 363.73
• DOI: 10.1016/j.envpol.2017.07.042
• Languages: English
• ISSNs: 0269-7491

Abstract

Abstract: Exposure experiments were conducted to evaluate the influence of dissolved organic matter (DOM) on the toxicity of ZnO-NPs (10–30 nm) and dissolved Zn at sub-lethal doses (50 and 5 ppm, respectively) to zebrafish ( Danio rerio ). Humic acid, alginic acid, bovine serum albumin and various natural DOM isolated from rivers as the Milwaukee River-WI (NOMW), Yukon River-AK (NOMA) and Suwannee River-GA DOM (NOMS) were used to represent humic substances (HA), carbohydrates (CHO), proteins (PTN), and natural organic matter (NOM), respectively. Initial experiments were carried out to confirm the toxic effect of ZnO-NPs at 50 ppm, followed by mitigation experiments with different types and concentrations of DOM (0.4–40 mg-C/L). Compared to 0% hatch of 50 ppm ZnO-NPs exposed embryos at 72 h post fertilization (hpf), NOMS, NOMW and HA had the best mitigative effects on hatching (53–65%), followed by NOMA, CHO and PTN (19–35%); demonstrating that the mitigation effects on ZnO-NPs toxicity were related to DOM's quantity and composition. At 96 hpf, 20% of embryos exposed to 50 ppm ZnO-NPs hatched, 100% of embryos reared in embryo medium hatched, and close to 100% of the embryos hatched upon mitigation, except for those mitigated with PTN which had less effect. Dissolved Zn (5 ppm) also exhibited the same toxicity on embryos as ZnO-NPs (50 ppm). However, in the presence of HA, NOM and CHO, the hatching rates at 72 and 96 hpf increased significantly compared to 5% hatch without DOM. The overall mitigation effects produced by DOM followed the order of HA ≥ NOMS > NOM (A&W) > CHO >> PTN, although specific mitigation effects varied with DOM concentration and functionalities. Our results also indicate that the toxicity of ZnO-NPs to embryos was mostly derived from NPs although dissolved Zn released from ZnO-NPs also interacted with embryos, affecting hatching, but to a less extent. Graphical abstract: Highlights: ZnO-NPs toxicity in embryonic zebrafish was examined in the presence/absence of DOM. Toxicity to the zebrafish was mainly from ZnO-NPs and not dissolved Zn. Natural DOM modified the surface properties/stability of ZnO-NPs. Natural DOM can mitigate the toxicity caused by ZnO-NPs and high levels of dissolved Zn. Humic acids were the most effective mitigators of ZnO-NPs and dissolved Zn toxicity.