Comparative toxicity and endocrine disruption potential of urban and rural atmospheric organic PM1 in JEG-3 human placental cells. (November 2017)

• Record Type: Journal Article
• Authors: van Drooge, Barend L.; Marqueño, Anna; Grimalt, Joan O.; Fernández, Pilar; Porte, Cinta
• Is Part Of: Environmental pollution. Volume 230(2017)
• Page Start: 378
• Page End: 386
• Publication Date: 2017-11
• Subjects: PM1 -- Organic air pollution -- Biomass burning -- Human placental JEG 3 cells -- Cytotoxicity -- Endocrine disruption; Pollution -- Periodicals; Pollution -- Environmental aspects -- Periodicals; Environmental Pollution -- Periodicals; Pollution -- Périodiques; Pollution -- Aspect de l'environnement -- Périodiques; Pollution -- Effets physiologiques -- Périodiques; Pollution; Pollution -- Environmental aspects; Periodicals; Electronic journals; 363.73
• DOI: 10.1016/j.envpol.2017.06.025
• Languages: English
• ISSNs: 0269-7491

Abstract

Abstract: Outdoor ambient air particulate matter and air pollution are related to adverse effects on human health. The present study assesses the cytotoxicity and ability to disrupt aromatase activity of organic PM1 extracts from rural and urban areas at equivalent air volumes from 2 to 30 m 3, in human placental JEG-3 cells. Samples were chemically analyzed for particle bounded organic compounds with endocrine disrupting potential, i.e. PAH, O-PAH, phthalate esters, but also for organic molecular tracer compounds for the emission source identification. Rural samples collected in winter were cytotoxic at the highest concentration tested and strongly inhibited aromatase activity in JEG-3 cells. No cytotoxicity was detected in summer samples from the rural site and the urban samples, while aromatase activity was moderately inhibited in these samples. In the urban area, the street site samples, collected close to intensive traffic, showed stronger inhibition of aromatase activity than the samples simultaneously collected at a roof site, 50 m above ground level. The cytotoxicity and endocrine disruption potential of the samples were linked to combustion products, i.e. PAH and O-PAH, especially from biomass burning in the rural site in winter. Graphical abstract: Highlights: Organic extracts of outdoor ambient air PM1 showed aromatase activity inhibition in exposed human placental JEG-3 cells. Cytotoxicity and strongest endocrine disruption was observed in rural winter samples, while lowest inhibition was observed in urban background site 50 m above a busy street. Cytotoxicity and aromatase activity inhibition in the samples were linked to combustion products, i.e. PAH and O-PAH, especially from biomass burning. Abstract : Organic extracts from ambient air PM1 related to biomass burning are more cytotoxic and have stronger endocrine disruption potential than urban PM1 .