A highly selective two-photon probe with large turn-on signal for imaging endogenous HOCl in living cells. (November 2017)

• Record Type: Journal Article
• Authors: Cao, Jingjing; Jiang, Dong-Ming; Ren, Xin; Li, Ting; Gong, Xiao-Ting; Yang, Yong-Rui; Xu, Zhu-Guo; Sun, Chun-Lin; Shi, Zi-Fa; Zhang, Shengxiang; Zhang, Hao-Li
• Is Part Of: Dyes and pigments. Volume 146(2017)
• Page Start: 279
• Page End: 286
• Publication Date: 2017-11
• Subjects: Two-photon fluorescent probe -- Reactive oxygen species -- Hypochlorous acid -- Endogenous bio-imaging; Dyes and dyeing -- Periodicals; Pigments -- Periodicals; 667.2
• DOI: 10.1016/j.dyepig.2017.07.006
• Languages: English
• ISSNs: 0143-7208

Abstract

Abstract: Hypochlorous acid (HOCl) is one of the typical reactive oxygen species (ROS), which plays a crucial role in the immune system, and is involved in many neurodegenerative disorders, such as ischemic stroke, Alzheimer's, and Parkinson's disease. A two-photon probe for sensing HOCl with high selectivity, fast response (within 30 s), good penetration depth and low cytotoxicity is reported. Under two-photon excitation, the probe exhibited a remarkable fluorescence ''turn-on'' response to HOCl. Furthermore, the probe was successfully applied to the monitoring of endogenous HOCl stimulated by lipopolysaccharide in living microglia BV-2 cells. Graphical abstract: Highlights: Large two-photon action cross-section of 1131.5 GM with small molecular weight. High specificity, fast response for HOCl fluorescence monitoring in a broad pH range. Selective imaging of endogenous HOCl in microglia BV-2 cells with low cytotoxicity.