Increased serum miR-7 is a promising biomarker for type 2 diabetes mellitus and its microvascular complications. (August 2017)

• Record Type: Journal Article
• Authors: Wan, Shujun; Wang, Jun; Wang, Jing; Wu, Jia; Song, Jiaxi; Zhang, Chen-Yu; Zhang, Chunni; Wang, Cheng; Wang, Jun-Jun
• Is Part Of: Diabetes research and clinical practice. Volume 130(2017)
• Page Start: 171
• Page End: 179
• Publication Date: 2017-08
• Subjects: Serum microRNA -- miR-7 -- Type 2 diabetes -- Microvascular complications -- Biomarkers; Diabetes -- Periodicals; Diabetes Mellitus -- Periodicals; 616.462
• DOI: 10.1016/j.diabres.2017.06.005
• Languages: English
• ISSNs: 0168-8227

Abstract

Highlights: A case-control study was conducted to characterize serum miR-7 signature in T2DM. Serum miR-7 were significantly higher in the T2DM and T2DMC patients than controls. Increased serum miR-7 is novel marker for T2DM and its microvascular complications. Abstract: Aims: To investigate the alteration pattern and physiologic state of islet-specific miR-7 in the serum of patients with type 2 diabetes mellitus (T2DM) and T2DM-associated microvascular complications (T2DMC) and to evaluate its clinical significance. Methods: The levels of serum miR-7 were firstly examined and compared in 76 T2DM patients, 76 T2DMC patients and 74 age-gender matched controls using RT-qPCR. Subsequently, the physiologic state of serum miR-7 was characterized by determining its concentrations in isolated exosomes and corresponding exosome-free samples from the same three cohorts' samples. Moreover, statistical analyzes were performed to evaluate the associations of serum miR-7 with T2DM and T2DMC. Results: Serum miR-7 was significantly elevated in the T2DM patients [(401.0 ± 34.37) fmol/L, P < 0.001] and in the T2DMC patients [(501.4 ± 81.69) fmol/L, P < 0.001] when compared with the controls [(175.7 ± 16.59) fmol/L]. Circulating miR-7 was mainly existed as exosome-free form rather than in membrane-bound exosomes. The concentrations of exosome-free miR-7 were markedly higher in the T2DM group [(107.2 ± 9.63) fmol/L, P < 0.001] and in the T2DMC group [(122.1 ± 10.80) fmol/L, P < 0.001] compared to the control group [(54.18 ± 2.37) fmol/L]. Logistic regression and ROC curve analyses revealed the serum miR-7 was significantly associated with T2DM and microvascular complications ( P < 0.05). Conclusion: Increased serum miR-7 might have the potential as a promising marker for T2DM and its microvascular complications.