A novel C-type lectin gene is a strong candidate gene for Benedenia disease resistance in Japanese yellowtail, Seriola quinqueradiata. (November 2017)

• Record Type: Journal Article
• Authors: Nakamoto, Masatoshi; Takeuchi, Yusuke; Akita, Kazuki; Kumagai, Ryo; Suzuki, Junpei; Koyama, Takashi; Noda, Tsutomu; Yoshida, Kazunori; Ozaki, Akiyuki; Araki, Kazuo; Sakamoto, Takashi
• Is Part Of: Developmental and comparative immunology. Volume 76(2017)
• Page Start: 361
• Page End: 369
• Publication Date: 2017-11
• Subjects: Japanese yellowtail -- Seriola quinqueradiata -- Benedenia disease -- Parasitic disease resistance -- C-type lectin -- Marker-assisted selection; Immunology -- Periodicals; Developmental immunology -- Periodicals; 616.079
• DOI: 10.1016/j.dci.2017.07.010
• Languages: English
• ISSNs: 0145-305X

Abstract

Abstract: Little is known about mechanisms of resistance to parasitic diseases in marine finfish. Benedenia disease is caused by infection by the monogenean parasite Benedenia seriolae . Previous quantitative trait locus (QTL) analyses have identified a major QTL associated with resistance to Benedenia disease in linkage group Squ2 of the Japanese yellowtail/amberjack Seriola quinqueradiata. To uncover the bioregulatory mechanism of Benedenia disease resistance, complete Illumina sequencing of BAC clones carrying genomic DNA for the QTL region in linkage group Squ2 was performed to reveal a novel C-type lectin in this region. Expression of the mRNA of this C-type lectin was detected in skin tissue parasitized by B. seriolae . Scanning for single nucleotide polymorphisms (SNPs) uncovered a SNP in the C-type lectin/C-type lectin-like domain that was significantly associated with B. seriolae infection levels. These results strongly suggest that the novel C-type lectin gene controls resistance to Benedenia disease in Japanese yellowtails. Highlights: The QTL region for Benedenia disease resistance was completely sequenced. A novel C-type lectin was located in this QTL region. A SNP located in the CTL domain was associated with parasite infection levels.