Differential expression and functional roles of chemokine (C-C motif) ligand 23 and its receptor chemokine (C-C motif) receptor type 1 in the uterine endometrium during early pregnancy in pigs. (November 2017)

• Record Type: Journal Article
• Authors: Jeong, Wooyoung; Bae, Hyocheol; Lim, Whasun; Bazer, Fuller W.; Song, Gwonhwa
• Is Part Of: Developmental and comparative immunology. Volume 76(2017)
• Page Start: 316
• Page End: 325
• Publication Date: 2017-11
• Subjects: CCL23 -- CCR1 -- Endometrium -- Cell proliferation -- ER stress -- Intracellular signaling; Immunology -- Periodicals; Developmental immunology -- Periodicals; 616.079
• DOI: 10.1016/j.dci.2017.07.007
• Languages: English
• ISSNs: 0145-305X

Abstract

Abstract: Many chemokines expressed by cells of the uterine endometrium of mammals are involved in cell-cell interactions. However, little is known about expression and functional roles of chemokine (C-C motif) ligand 23 (CCL23) in the uterine endometrium. Results of this study demonstrated that CCL23 and its receptor, chemokine (C-C motif) receptor type 1 (CCR1), are up-regulated in porcine endometria during pregnancy. CCL23 and CCR1 mRNAs were strongly expressed in endometrial glandular (GE) and luminal (LE) epithelial cells. Treatment of porcine uterine luminal epithelial (pLE) cells with recombinant CCL23 increased the abundances of PCNA and cyclin D1, and enhanced proliferation and cell cycle progression in pLE cells. CCL23 also stimulated phosphorylation of cell signaling molecules including AKT and MAPKs in pLE cells. Furthermore, ER stress-related molecules were reduced by CCL23. These results suggest that CCL23-CCR1 signaling is important for endometrial development and establishment of pregnancy in pigs. Highlights: CCL23-CCR1 system is up-regulated in the uterine endometrium during early pregnancy. CCL23 induces the proliferation and cell cycle progression of pLE cells. CCL23 activates intracellular signaling pathways including PI3K/AKT and MAPK. CCL23 reduces tunicamycin-induced ER stress in pLE cells. CCL23-CCR1 is important for development of the endometrium during early pregnancy.