Characterization of immune response in Staphylococcus aureus chronically infected bovine mammary glands during active involution. (October 2017)

• Record Type: Journal Article
• Authors: Andreotti, Carolina S.; Baravalle, Celina; Sacco, Sofía C.; Lovato, Melisa; Pereyra, Elizabet A.L.; Renna, María S.; Ortega, Hugo H.; Calvinho, Luis F.; Dallard, Bibiana E.
• Is Part Of: Comparative immunology, microbiology and infectious diseases. Volume 54(2017)
• Page Start: 51
• Page End: 60
• Publication Date: 2017-10
• Subjects: Immune response -- Staphylococcus aureus -- Mastitis -- Involution; Communicable diseases in animals -- Periodicals; Veterinary immunology -- Periodicals; Veterinary microbiology -- Periodicals; Immunology -- Periodicals; Microbiology -- Periodicals; Communicable diseases -- Periodicals; Communicable Diseases -- immunology -- Periodicals; Communicable Diseases -- veterinary -- Periodicals; Allergy and Immunology -- Periodicals; Microbiology -- Periodicals; Veterinary Medicine -- Periodicals; Immunologie -- Périodiques; Microbiologie -- Périodiques; Maladies infectieuses -- Périodiques; Communicable diseases; Immunology; Microbiology; Electronic journals; Periodicals; 636.08969
• DOI: 10.1016/j.cimid.2017.08.005
• Languages: English
• ISSNs: 0147-9571

Abstract

Highlights: Protein expression of TLR2 and 4 was increased in chronically S. aureus -infected mammary glands. TNF-α, IL-1α and IL-17 protein expression were increased in chronically S. aureus -infected glands. Number of macrophages, T and B cells were increased in chronically S. aureus -infected glands. Chronic S. aureus IMI induced a sustained and exacerbated immune response during involution. Abstract: The aim of this study was to characterize the immune response in Staphylococcus aureus chronically infected bovine mammary glands during active involution. Twenty-one Holstein non-pregnant cows in late lactation either uninfected or with chronic naturally acquired S. aureus intramammary infections (IMI) were included in this study. Cows were slaughtered at 7, 14 and 21 d after cessation of milking and samples for immunohistochemical analysis were taken. Protein expression of toll-like receptor 2 (TLR2) and TLR4 was significantly higher in S. aureus -infected quarters than in uninfected controls at the three involution stages studied. Protein expression of tumor necrosis factor-alpha (TNF-α), interleukin (IL)-1α and IL-17 was significantly affected by IMI; being higher in S. aureus -infected than uninfected quarters during all evaluated stages. In S. aureus -infected and uninfected quarters protein expression of lactoferrin increased from day 7–14 of involution, decreasing significantly to day 21 in mammary quarters with chronic infections. The number of monocytes-macrophages was significantly higher in S. aureus -infected than in uninfected control quarters at 7 and 21 d of involution. The number of T lymphocytes was significantly higher in S. aureus -infected than in uninfected quarters at 7 and 14 d of involution while the number of B lymphocytes was significantly higher in S. aureus -infected than in uninfected quarters during all evaluated stages, showing a progressive increase as involution advanced. These results demonstrated a sustained and exacerbated innate and adaptive immune response during chronic S. aureus IMI, playing a critical role in the infection control during active involution.