Mutagenic, recombinogenic and carcinogenic potential of thiamethoxam insecticide and formulated product in somatic cells of Drosophila melanogaster. (November 2017)

• Record Type: Journal Article
• Authors: de Morais, Cássio Resende; Carvalho, Stephan Malfitano; Carvalho Naves, Maria Paula; Araujo, Galber; de Rezende, Alexandre Azenha Alves; Bonetti, Ana Maria; Spanó, Mário Antônio
• Is Part Of: Chemosphere. Volume 187(2017)
• Page Start: 163
• Page End: 172
• Publication Date: 2017-11
• Subjects: Actara -- Insecticide -- Neonicotinoid -- SMART -- Warts; Pollution -- Periodicals; Pollution -- Physiological effect -- Periodicals; Environmental sciences -- Periodicals; Atmospheric chemistry -- Periodicals; 551.511
• DOI: 10.1016/j.chemosphere.2017.08.108
• Languages: English
• ISSNs: 0045-6535

Abstract

Abstract: Thiamethoxam (TMX) belongs to a class of neuro-active insecticides referred as neonicotinoids, while actara ® (AC) is one of the most popular TMX-based products in Brazil. The aim of this study was to evaluate the mutagenic, recombinogenic and carcinogenic potential of TMX and AC insecticides. The mutagenic and recombinogenic effect of TMX and AC were evaluated in vivo by the Somatic Mutation and Recombination Test (SMART) while carcinogenic effects were evaluated through the Test for Detection of Epithelial Tumor Clones (wts test), both in somatic cells of Drosophila melanogaster. In the SMART, third instar larvae from standard (ST) and high bioactivation (HB) crosses were treated with different concentrations of TMX and AC (2.4; 4.8; 9.7 × 10 −4 mM and 1.9 × 10 −3 mM). The results revealed mutagenic effects at the highest concentrations tested in the HB cross. In the test for the detection of epithelial tumor, third instar larvae resulting from the cross between wts/TM3, Sb 1 virgin females and mwh/mwh males were treated with the same concentrations of TMX and AC used in the SMART. No carcinogenic effect was observed at any of the concentrations tested. In this work, the inhibition of the mechanism of repair by homologous recombination was observed in flies exposed to 9.7 × 10 −4 and 1.9 × 10 −3 mM of AC. In conclusion, TMX and AC demonstrated to be a promutagen in the highest concentrations tested. Highlights: The mutagenic, recombinogenic and carcinogenic potential of Thiametoxam and Actara were studied in vivo . Thiametoxam and Actara are mutagenic after bioactivation. Increasing the metabolic capacity decreases the toxic effect of Thiametoxam and Actara. Inert ingredients present in Actara may interfere with the repair mechanism by homologous recombination.