Kinetic and molecular analyses reveal isoprene degradation potential of Methylobacterium sp. (October 2017)

• Record Type: Journal Article
• Authors: Srivastva, Navnita; Vishwakarma, P.; Bhardwaj, Y.; Singh, A.; Manjunath, K.; Dubey, Suresh K.
• Is Part Of: Bioresource technology. Volume 242(2017)
• Page Start: 87
• Page End: 91
• Publication Date: 2017-10
• Subjects: Isoprene -- Biodegradation -- Methylobacterium -- Kinetics -- FTIR; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2017.02.002
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: Methylobacterium sp. PV1 was isolated from uncontaminated agricultural field. Isoprene degradation by Methylobacterium sp. PV1 was assessed in batch mode. Efficient degradation up to 150 ppm was observed with 80% degradation. Kinetic parameters for degradation were calculated using Michaelis-Menten model. Abstract: Efforts were made to isolate and characterize bacteria capable of growing on methane and organic compounds, and to achieve the simultaneous degradation of more than one pollutant. Among the methanotrophs, species of Methylobacterium was able to catabolize a variety of hydrocarbons, including the branched-chain alkenes. Therefore, laboratory incubations experiments were carried out in batch mode to assess the potential of Methylobacterium sp. PV1 for degrading isoprene, the low-molecular-weight alkene, the most abundant non-methane volatile hydrocarbon present in the environment. Methylobacterium sp. PV1, isolated from paddy field soil, was characterized by pmoA and 16S rRNA gene sequencing and FAME analysis, and used for isoprene degradation. The kinetics of biodegradation is studied using the Michaelis-Menten model. The optimum degradation (80%) with maximum average relative degradation rate was observed at 150 ppm isoprene. The degradation products were also analyzed using FTIR.